CIS Display: DNA-based technology as a platform for discovery of therapeutic biologics (Invited Contribution)

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

CIS display is a cell-free in vitro display technology, based on linear dsDNA templates. This platform allowed for the evolution of peptides, antibody fragments, and protein scaffolds, mainly for therapeutic applications. In this chapter, CIS display application for discovery of therapeutics biologics is revised. In addition, an improved protocol for DNA recovery at the end of each selection round is described. This improved protocol allows to streamline selections using CIS display, thus accelerating the therapeutic discovery of novel biologics relevant for the pharmaceutical industry.
Original languageEnglish
Title of host publicationPeptide and Protein Engineering: From Concepts to Biotechnological Applications
EditorsOlga Iranzo, Ana Cecília Roque
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Chapter10
Pages173-188
Edition1
ISBN (Electronic)978-1-0716-0720-6
ISBN (Print)978-1-0716-0719-0
DOIs
Publication statusPublished - 2020
EventPepperSchool 2018 (1st edition) - Carry le Rouet, Marseille, France
Duration: 14 May 201817 May 2018
Conference number: 1
https://eventos.fct.unl.pt/pepper_school/pages/home

Publication series

NameSpringer Protocols Handbooks
PublisherHumana Press Inc.
ISSN (Print)1949-2448
ISSN (Electronic)1949-2456

Workshop

WorkshopPepperSchool 2018 (1st edition)
CountryFrance
CityMarseille
Period14/05/1817/05/18
Internet address

Keywords

  • CIS display
  • In vitro evolution
  • Protein scaffolds
  • Antibody fragments
  • Therapeutics
  • Peptides

Fingerprint

Dive into the research topics of 'CIS Display: DNA-based technology as a platform for discovery of therapeutic biologics (Invited Contribution)'. Together they form a unique fingerprint.

Cite this