TY - JOUR
T1 - Chromobacterium violaceum: Important Insights for Virulence and Biotechnological Potential by Exoproteomic Studies
AU - Carepo, Marta Sofia Peixe
N1 - Sem PDF
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Chromobacterium violaceum is a beta-proteobacterium with high biotechnological potential, found in tropical environments. This bacterium causes opportunistic infections in both humans and animals, that can spread throughout several tissues, quickly leading to the death of the host. Genomic studies identified potential mechanisms of pathogenicity but no further studies were done to confirm the expression of these systems. In this study 36 unique protein entries were identified in databank from a two-dimensional profile of C. violaceum secreted proteins. Chromobacterium violaceum exoproteomic preliminary studies confirmed the production of proteins identified as virulence factors (such as a collagenase, flagellum proteins, metallopeptidases, and toxins), allowing us to better understand its pathogenicity mechanisms. Biotechnologically interesting proteins (such as chitinase and chitosanase) were also identified among the secreted proteins, as well as proteins involved in the transport and capture of amino acids, carbohydrates, and oxidative stress protection. Overall, the secreted proteins identified provide us important insights on pathogenicity mechanisms, biotechnological potential, and environment adaptation of C. violaceum.
AB - Chromobacterium violaceum is a beta-proteobacterium with high biotechnological potential, found in tropical environments. This bacterium causes opportunistic infections in both humans and animals, that can spread throughout several tissues, quickly leading to the death of the host. Genomic studies identified potential mechanisms of pathogenicity but no further studies were done to confirm the expression of these systems. In this study 36 unique protein entries were identified in databank from a two-dimensional profile of C. violaceum secreted proteins. Chromobacterium violaceum exoproteomic preliminary studies confirmed the production of proteins identified as virulence factors (such as a collagenase, flagellum proteins, metallopeptidases, and toxins), allowing us to better understand its pathogenicity mechanisms. Biotechnologically interesting proteins (such as chitinase and chitosanase) were also identified among the secreted proteins, as well as proteins involved in the transport and capture of amino acids, carbohydrates, and oxidative stress protection. Overall, the secreted proteins identified provide us important insights on pathogenicity mechanisms, biotechnological potential, and environment adaptation of C. violaceum.
KW - CORYNEBACTERIUM-PSEUDOTUBERCULOSIS
KW - GENE
KW - SECRETED PROTEINS
KW - ESCHERICHIA-COLI
KW - BINDING PROTEINS
KW - PROTEOMICS
KW - BIOSYNTHESIS
KW - ELONGATION-FACTOR-TU
KW - LEGIONELLA-PNEUMOPHILA
KW - CLONING
U2 - 10.1007/s00284-013-0334-5
DO - 10.1007/s00284-013-0334-5
M3 - Article
C2 - 23455494
SN - 0343-8651
VL - 67
SP - 100
EP - 106
JO - Current Microbiology
JF - Current Microbiology
IS - 1
ER -