Cholesterol is Inefficiently Converted to Cholesteryl Esters in the Blood of Cardiovascular Disease Patients

Mathias J. Gerl, Winchil L.C. Vaz, Neuza Domingues, Christian Klose, Michal A. Surma, Júlio L. Sampaio, Manuel S. Almeida, Gustavo Rodrigues, Pedro Araújo-Gonçalves, Jorge Ferreira, Claudia Borbinha, João Pedro Marto, Miguel Viana-Baptista, Kai Simons, Otilia V. Vieira

Research output: Contribution to journalArticle

Abstract

Shotgun lipidomic analysis of 203 lipids in 13 lipid classes performed on blood plasma of donors who had just suffered an acute coronary syndrome (ACS, n = 74), or an ischemic stroke (IS, n = 21), or who suffer from stable angina pectoris (SAP, n = 78), and an age-matched control cohort (n = 52), showed some of the highest inter-lipid class correlations between cholesteryl esters (CE) and phosphatidylcholines (PC) sharing a common fatty acid. The concentration of lysophospatidylcholine (LPC) and ratios of concentrations of CE to free cholesterol (Chol) were also lower in the CVD cohorts than in the control cohort, indicating a deficient conversion of Chol to CE in the blood plasma in the CVD subjects. A non-equilibrium reaction quotient, Q′, describing the global homeostasis of cholesterol as manifested in the blood plasma was shown to have a value in the CVD cohorts (Q′ACS = 0.217 ± 0.084; Q′IS = 0.201 ± 0.084; Q′SAP = 0.220 ± 0.071) that was about one third less than in the control cohort (Q′Control = 0.320 ± 0.095, p < 1 × 10−4), suggesting its potential use as a rapid predictive/diagnostic measure of CVD-related irregularities in cholesterol homeostasis.

LanguageEnglish
Article number14764
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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Hematologic Diseases
Cholesterol Esters
Cardiovascular Diseases
Cholesterol
Lipids
Homeostasis
Stable Angina
Firearms
Acute Coronary Syndrome
Blood Donors
Phosphatidylcholines
Fatty Acids
Stroke

Cite this

Gerl, Mathias J. ; Vaz, Winchil L.C. ; Domingues, Neuza ; Klose, Christian ; Surma, Michal A. ; Sampaio, Júlio L. ; Almeida, Manuel S. ; Rodrigues, Gustavo ; Araújo-Gonçalves, Pedro ; Ferreira, Jorge ; Borbinha, Claudia ; Marto, João Pedro ; Viana-Baptista, Miguel ; Simons, Kai ; Vieira, Otilia V. / Cholesterol is Inefficiently Converted to Cholesteryl Esters in the Blood of Cardiovascular Disease Patients. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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abstract = "Shotgun lipidomic analysis of 203 lipids in 13 lipid classes performed on blood plasma of donors who had just suffered an acute coronary syndrome (ACS, n = 74), or an ischemic stroke (IS, n = 21), or who suffer from stable angina pectoris (SAP, n = 78), and an age-matched control cohort (n = 52), showed some of the highest inter-lipid class correlations between cholesteryl esters (CE) and phosphatidylcholines (PC) sharing a common fatty acid. The concentration of lysophospatidylcholine (LPC) and ratios of concentrations of CE to free cholesterol (Chol) were also lower in the CVD cohorts than in the control cohort, indicating a deficient conversion of Chol to CE in the blood plasma in the CVD subjects. A non-equilibrium reaction quotient, Q′, describing the global homeostasis of cholesterol as manifested in the blood plasma was shown to have a value in the CVD cohorts (Q′ACS = 0.217 ± 0.084; Q′IS = 0.201 ± 0.084; Q′SAP = 0.220 ± 0.071) that was about one third less than in the control cohort (Q′Control = 0.320 ± 0.095, p < 1 × 10−4), suggesting its potential use as a rapid predictive/diagnostic measure of CVD-related irregularities in cholesterol homeostasis.",
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Cholesterol is Inefficiently Converted to Cholesteryl Esters in the Blood of Cardiovascular Disease Patients. / Gerl, Mathias J.; Vaz, Winchil L.C.; Domingues, Neuza; Klose, Christian; Surma, Michal A.; Sampaio, Júlio L.; Almeida, Manuel S.; Rodrigues, Gustavo; Araújo-Gonçalves, Pedro; Ferreira, Jorge; Borbinha, Claudia; Marto, João Pedro; Viana-Baptista, Miguel; Simons, Kai; Vieira, Otilia V.

In: Scientific Reports, Vol. 8, No. 1, 14764, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Vaz, Winchil L.C.

AU - Domingues, Neuza

AU - Klose, Christian

AU - Surma, Michal A.

AU - Sampaio, Júlio L.

AU - Almeida, Manuel S.

AU - Rodrigues, Gustavo

AU - Araújo-Gonçalves, Pedro

AU - Ferreira, Jorge

AU - Borbinha, Claudia

AU - Marto, João Pedro

AU - Viana-Baptista, Miguel

AU - Simons, Kai

AU - Vieira, Otilia V.

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