Chitosan-based vectors mediate long-term gene expression in the retina

Gabriela A. Silva, Ana V Oliveira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

One of the major limitations to the success of non-viral gene therapy has been the low gene transfer efficiency and short transgene expression. Chitosan has shown great promise as a non-viral carrier due to its biocompatibility, biodegradability and low cost, but limited by its low transfection efficiency. Our goal is to develop chitosan-based non-viral vectors optimized for retinal gene therapy and continued gene expression. The gene transfer efficiency and biocompatibility of chitosan-pDNA polyplexes was evaluated both in vitro on retinal pigment epithelial (ARPE-19) cells and human embryonic kidney (HEK293T) cells, and in vivo in the mouse retina. Our results show that chitosan polyplexes have size and surface charge consistent with gene delivery. When considering off the shelf applications, vector stability is crucial, and our vectors are stable both in storage (4 °C) and physiological conditions (37 °C), an d remain stable after several freeze-th aw cycles. Despite moderate in vitro efficiency, in vivo sub-retinal adm inistration shows sust ained transgene expression in RPE cells at least 6 months post-injection. Thes e result i t fs show that chitosan-based vectors can successfully transfer genes to the retina and provide persistent transgene expression.

Original languageEnglish
Pages (from-to)373-382
Number of pages10
JournalJournal of Bionanoscience
Volume9
Issue number5
DOIs
Publication statusPublished - 1 Oct 2015

Keywords

  • Chitosan
  • Gene delivery
  • Retina

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