Abstract

Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.

Original languageEnglish
Pages (from-to)455-464
Number of pages10
JournalCarbohydrate Polymers
Volume130
DOIs
Publication statusPublished - 5 Oct 2015

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Chitin
Glucans
Polymers
Biopolymers
Cosmetics
Deionized water
Polydispersity
Washing
X-Ray Diffraction
Biomass
Drug products
Raw materials
Salts
Molecular Weight
Molecular weight
Nuclear magnetic resonance
Decomposition
Proteins
Food
X ray diffraction

Keywords

  • Chitin-glucan complex (CGC)
  • Komagataella pastoris
  • Molecular weight
  • Structural analysis
  • Thermal properties

Cite this

@article{0f54385c6399495a80efb2285bfc2ee7,
title = "Chitin-glucan complex production by Komagataella pastoris: Downstream optimization and product characterization",
abstract = "Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt{\%}, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50{\%}. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.",
keywords = "Chitin-glucan complex (CGC), Komagataella pastoris, Molecular weight, Structural analysis, Thermal properties",
author = "In{\^e}s Farinha and Paulo Duarte and Ana Pimentel and Evgeniya Plotnikova and B{\'a}rbara Chagas and Lu{\'i}s Mafra and Christian Grandfils and Freitas, {Maria Filomena Andrade de} and Elvira Fortunato and Reis, {Maria D'ascens{\~a}o Carvalho Fernandes Miranda}",
note = "This project was financially supported by 73100 Lda. under project {"}Biopolymers Production from Glycerol{"}, 2010/2013. The work was financed by the European projects INVISIBLE (FP7 ERC AdG no 228144) and ORAMA CP-IP 246334-2, APPLE (FP7-NMP-2010-SME/262782-2), and by Fundacao para a Ciencia e Tecnologia (FC&T, Portugal), through projects PEst-C/EQB/LA0006/2013 and PEst-C/CTM/LA0025/2013-14. Ines Farinha acknowledges FC&T and Pharma73 S.A. fellowship SFRH/BDE/52089/2013. Barbara Chagas, Filomena Freitas and Ana Pimentel acknowledge FC&T fellowships SFRH/BD/44988/2008, SFRH/BPD/72280/2010 and SFRH/BPD/76992/2011, respectively. Evgeniya Plotnikova was granted by WBI for a PhD fellowship at CEIB, University of Liege.",
year = "2015",
month = "10",
day = "5",
doi = "10.1016/j.carbpol.2015.05.034",
language = "English",
volume = "130",
pages = "455--464",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Chitin-glucan complex production by Komagataella pastoris: Downstream optimization and product characterization

AU - Farinha, Inês

AU - Duarte, Paulo

AU - Pimentel, Ana

AU - Plotnikova, Evgeniya

AU - Chagas, Bárbara

AU - Mafra, Luís

AU - Grandfils, Christian

AU - Freitas, Maria Filomena Andrade de

AU - Fortunato, Elvira

AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda

N1 - This project was financially supported by 73100 Lda. under project "Biopolymers Production from Glycerol", 2010/2013. The work was financed by the European projects INVISIBLE (FP7 ERC AdG no 228144) and ORAMA CP-IP 246334-2, APPLE (FP7-NMP-2010-SME/262782-2), and by Fundacao para a Ciencia e Tecnologia (FC&T, Portugal), through projects PEst-C/EQB/LA0006/2013 and PEst-C/CTM/LA0025/2013-14. Ines Farinha acknowledges FC&T and Pharma73 S.A. fellowship SFRH/BDE/52089/2013. Barbara Chagas, Filomena Freitas and Ana Pimentel acknowledge FC&T fellowships SFRH/BD/44988/2008, SFRH/BPD/72280/2010 and SFRH/BPD/76992/2011, respectively. Evgeniya Plotnikova was granted by WBI for a PhD fellowship at CEIB, University of Liege.

PY - 2015/10/5

Y1 - 2015/10/5

N2 - Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.

AB - Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.

KW - Chitin-glucan complex (CGC)

KW - Komagataella pastoris

KW - Molecular weight

KW - Structural analysis

KW - Thermal properties

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U2 - 10.1016/j.carbpol.2015.05.034

DO - 10.1016/j.carbpol.2015.05.034

M3 - Article

VL - 130

SP - 455

EP - 464

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -