TY - JOUR
T1 - Chitin-glucan complex production by Komagataella pastoris: Downstream optimization and product characterization
AU - Farinha, Inês
AU - Duarte, Paulo
AU - Pimentel, Ana
AU - Plotnikova, Evgeniya
AU - Chagas, Bárbara
AU - Mafra, Luís
AU - Grandfils, Christian
AU - Freitas, Maria Filomena Andrade de
AU - Fortunato, Elvira
AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda
N1 - This project was financially supported by 73100 Lda. under project "Biopolymers Production from Glycerol", 2010/2013. The work was financed by the European projects INVISIBLE (FP7 ERC AdG no 228144) and ORAMA CP-IP 246334-2, APPLE (FP7-NMP-2010-SME/262782-2), and by Fundacao para a Ciencia e Tecnologia (FC&T, Portugal), through projects PEst-C/EQB/LA0006/2013 and PEst-C/CTM/LA0025/2013-14. Ines Farinha acknowledges FC&T and Pharma73 S.A. fellowship SFRH/BDE/52089/2013. Barbara Chagas, Filomena Freitas and Ana Pimentel acknowledge FC&T fellowships SFRH/BD/44988/2008, SFRH/BPD/72280/2010 and SFRH/BPD/76992/2011, respectively. Evgeniya Plotnikova was granted by WBI for a PhD fellowship at CEIB, University of Liege.
PY - 2015/10/5
Y1 - 2015/10/5
N2 - Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.
AB - Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.
KW - Chitin-glucan complex (CGC)
KW - Komagataella pastoris
KW - Molecular weight
KW - Structural analysis
KW - Thermal properties
UR - http://www.scopus.com/inward/record.url?scp=84930929220&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2015.05.034
DO - 10.1016/j.carbpol.2015.05.034
M3 - Article
C2 - 26076647
AN - SCOPUS:84930929220
SN - 0144-8617
VL - 130
SP - 455
EP - 464
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -