Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

M. Dionisio, L. Braz, M. Corvo, J. P. Lourenço, A. Grenha, A.m. Rosa Da Costa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation
Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume86
DOIs
Publication statusPublished - 1 May 2016

Fingerprint

Polyelectrolytes
Complexation
Derivatives
Fourier Transform Infrared Spectroscopy
Ammonium Compounds
Nuclear magnetic resonance
Nanoparticles
Sulfates
Polysaccharides
Salts
Drug delivery
Substitution reactions
Pharmaceutical Preparations
Proteins
Chemical analysis
pullulan
Experiments
glycidyl trimethylammonium

Keywords

  • (3-6): Sulfated pullulan
  • Tetraalkylammonium pullulan
  • Polyelectrolyte complexation

Cite this

Dionisio, M. ; Braz, L. ; Corvo, M. ; Lourenço, J. P. ; Grenha, A. ; Rosa Da Costa, A.m. / Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation. In: International Journal of Biological Macromolecules. 2016 ; Vol. 86. pp. 129-138.
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abstract = "Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation",
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Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation. / Dionisio, M.; Braz, L.; Corvo, M.; Lourenço, J. P.; Grenha, A.; Rosa Da Costa, A.m.

In: International Journal of Biological Macromolecules, Vol. 86, 01.05.2016, p. 129-138.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

AU - Dionisio, M.

AU - Braz, L.

AU - Corvo, M.

AU - Lourenço, J. P.

AU - Grenha, A.

AU - Rosa Da Costa, A.m.

N1 - Sem PDF. National Portuguese funding through FCT-Fundacao para a Ciencia e a Tecnologia (PTDC/SAU-FCF/100291/2008; PEst-OE/EQB/LA0023/2013; PEst-OE/QUI/UI4023/2011; UID/Multi/04378/2013,UID/CTM/50025/2013) FEDER funds through the COMPETE Programme Fundacao para a Ciencia e a Tecnologia Portuguese National NMR Network (RNRMN)

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N2 - Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation

AB - Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation

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