Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

M. Dionisio; L. Braz; M. Corvo; J. P. Lourenço; A. Grenha; A.m. Rosa Da Costa

doi:10.1016/j.ijbiomac.2016.01.054

Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

M. Dionisio, L. Braz, M. Corvo, J. P. Lourenço, A. Grenha, A.m. Rosa Da Costa

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation

Original language	English
Pages (from-to)	129-138
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	86
DOIs	https://doi.org/10.1016/j.ijbiomac.2016.01.054
Publication status	Published - 1 May 2016

Keywords

(3-6): Sulfated pullulan
Tetraalkylammonium pullulan
Polyelectrolyte complexation

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10.1016/j.ijbiomac.2016.01.054Licence: CC BY-NC-ND

http://linkinghub.elsevier.com/retrieve/pii/S0141813016300563

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Dionisio, M., Braz, L., Corvo, M., Lourenço, J. P., Grenha, A., & Rosa Da Costa, A. M. (2016). Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation. International Journal of Biological Macromolecules, 86, 129-138. https://doi.org/10.1016/j.ijbiomac.2016.01.054

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abstract = "Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation",

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AU - Braz, L.

AU - Corvo, M.

AU - Lourenço, J. P.

AU - Grenha, A.

AU - Rosa Da Costa, A.m.

N1 - Sem PDF. National Portuguese funding through FCT-Fundacao para a Ciencia e a Tecnologia (PTDC/SAU-FCF/100291/2008; PEst-OE/EQB/LA0023/2013; PEst-OE/QUI/UI4023/2011; UID/Multi/04378/2013,UID/CTM/50025/2013) FEDER funds through the COMPETE Programme Fundacao para a Ciencia e a Tecnologia Portuguese National NMR Network (RNRMN)

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N2 - Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation

AB - Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3 center dot DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on H-1- and C-13 NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation

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KW - Tetraalkylammonium pullulan

KW - Polyelectrolyte complexation

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JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

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