@inbook{11faee2767a242eead265e9aadfc825e,
title = "Characterization of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough",
abstract = "The [NiFeSe] hydrogenases are a subgroup of the well-characterized family of [NiFe] hydrogenases, in which a selenocysteine is a ligand to the nickel atom in the binuclear NiFe active site instead of cysteine. These enzymes display very interesting catalytic properties for biological hydrogen production and bioelectrochemical applications: high H2 production activity, bias for H2 evolution, low H2 inhibition, and some degree of O2 tolerance. Here we describe the methodologies employed to study the [NiFeSe] hydrogenase isolated from the sulfate-reducing bacteria D. vulgaris Hildenborough and the creation of a homologous expression system for production of variant forms of the enzyme.",
keywords = "Desulfovibrio vulgaris Hildenborough, Hydrogen, Infrared spectroscopy, Oxygen tolerance, Selenocysteine, Sulfate-reducing bacteria, X-ray crystallography, [NiFeSe] hydrogenases",
author = "S{\'o}nia Zacarias and Marisela V{\'e}lez and Marcos Pita and {De Lacey}, {Antonio L.} and Matias, {Pedro M.} and Pereira, {In{\^e}s A.C.}",
year = "2018",
month = jan,
day = "1",
doi = "10.1016/bs.mie.2018.10.003",
language = "English",
isbn = "9780128163610",
series = "Methods in Enzymology",
publisher = "Elsevier Academic Press Inc",
pages = "169--201",
editor = "Fraser Armstrong",
booktitle = "Methods in Enzymology",
}
