@article{e31b11b4327649c286225acbe03e973a,
title = "Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis",
abstract = "The study of human papillomavirus (HPV)-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. This study aimed to determine the HPV16 variant and sublineage present in the K14HPV16 mouse model. This information can be considered of great importance to further enhance this K14HPV16 model as an essential research tool and optimize its use for basic and translational studies. Our study evaluated HPV DNA from 17 samples isolated from 4 animals, both wild-type (n = 2) and HPV16-transgenic mice (n = 2). Total DNA was extracted from tissues and the detection of HPV16 was performed using a qPCR multiplex. HPV16-positive samples were subsequently whole-genome sequenced by next-generation sequencing techniques. The phylogenetic positioning clearly shows K14HPV16 samples clustering together in the sub-lineage A1 (NC001526.4). A comparative genome analysis of K14HPV16 samples revealed three mutations to the human papillomaviruses type 16 sublineage A1 representative strain. Knowledge of the HPV 16 variant is fundamental, and these findings will allow the rational use of this animal model to explore the role of the A1 sublineage in HPV-driven cancer.",
keywords = "carcinogenesis, HPV16, K14HPV16, lineage, variant",
author = "Daniela Cochicho and Alexandra Nunes and Gomes, {Jo{\~a}o Paulo} and Lu{\'i}s Martins and M{\'a}rio Cunha and Beatriz Medeiros-Fonseca and Paula Oliveira and Bastos, {Margarida M.S.M.} and Rui Medeiros and Joana Mendon{\c c}a and Luis Vieira and {Gil da Costa}, {Rui M.} and Ana Felix",
note = "by Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia/Minist{\'e}rio da Ci{\^e}ncia, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/ 04462/2020); from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Pro- Int. J. Mol. Sci. 2022, 23, 12371 8 of 10 gramme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT). This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundos Nacionais through the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics); and from LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); 2SMART (NORTE-01-0145- FEDER-000054), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). ",
year = "2022",
month = oct,
day = "15",
doi = "10.3390/ijms232012371",
language = "English",
volume = "23",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI - Multidisciplinary Digital Publishing Institute",
number = "20",
}