Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.
|Number of pages||4|
|Journal||Biochemical And Biophysical Research Communications|
|Publication status||Published - 30 Nov 2001|
- 3Fe-4S cluster
- Ferredoxin gene
- Iron-sulfur cluster
- Paramagnetic protein