Characterization of recombinant Desulfovibrio gigas ferredoxin

Pedro M. L. Rodrigues, Fernando Graça, Anjos L. Macedo, Isabel Moura, José J. G. Moura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.

Original languageEnglish
Pages (from-to)630-633
Number of pages4
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 30 Nov 2001


  • 3Fe-4S cluster
  • Ferredoxin
  • Ferredoxin gene
  • Iron-sulfur cluster
  • Paramagnetic protein


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