Characterization of recombinant Desulfovibrio gigas ferredoxin

Pedro M. L. Rodrigues; Fernando Graça; Anjos L. Macedo; Isabel Moura; José J. G. Moura

doi:10.1006/bbrc.2001.6016

Characterization of recombinant Desulfovibrio gigas ferredoxin

Pedro M. L. Rodrigues, Fernando Graça, Anjos L. Macedo, Isabel Moura, José J. G. Moura

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and ¹H NMR data of recombinant and wild-type protein are compared.

Original language	English
Pages (from-to)	630-633
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	289
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2001.6016
Publication status	Published - 30 Nov 2001

Keywords

3Fe-4S cluster
Ferredoxin
Ferredoxin gene
Iron-sulfur cluster
Paramagnetic protein

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10.1006/bbrc.2001.6016Licence: CC BY-NC-ND

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title = "Characterization of recombinant Desulfovibrio gigas ferredoxin",

abstract = "Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.",

keywords = "3Fe-4S cluster, Ferredoxin, Ferredoxin gene, Iron-sulfur cluster, Paramagnetic protein",

author = "Rodrigues, {Pedro M. L.} and Fernando Gra{\c c}a and Macedo, {Anjos L.} and Isabel Moura and Moura, {Jos{\'e} J. G.}",

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AU - Rodrigues, Pedro M. L.

AU - Graça, Fernando

AU - Macedo, Anjos L.

AU - Moura, Isabel

AU - Moura, José J. G.

N1 - This work was supported by PRAXIS XXI.

PY - 2001/11/30

Y1 - 2001/11/30

N2 - Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.

AB - Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.

KW - 3Fe-4S cluster

KW - Ferredoxin

KW - Ferredoxin gene

KW - Iron-sulfur cluster

KW - Paramagnetic protein

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U2 - 10.1006/bbrc.2001.6016

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EP - 633

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Characterization of recombinant Desulfovibrio gigas ferredoxin

Abstract

Keywords

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