Characterization of medium chain length polyhydroxyalkanoate produced from olive oil deodorizer distillate

Madalena V. Cruz, Diana Araújo, Vítor D. Alves, Filomena Freitas, Maria A M Reis

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21 Citations (Scopus)


Olive oil deodorizer distillate (OODD) was used for the first time as the sole substrate for polyhydroxyalkanoates (PHA) production by the bacterium Pseudomonas resinovorans in bioreactor cultivation. A PHA content in the biomass of 36±0.8wt% was attained within 19h of cultivation. A final polymer concentration of 4.7±0.3gL-1 was reached, corresponding to a volumetric productivity of 5.9±0.2gL-1day-1. The PHA was composed of 3-hydroxyoctanoate (48.3±7.3mol%), 3-hydroxydecanoate (31.6±2.6mol%), 3-hydroxyhexanoate (12.1±1.1mol%) and 3-hydroxydodecanoate (8.0±0.7mol%) and it had a glue-like consistency that did not solidify at room temperature. The polymer was highly amorphous, as shown by its low crystallinity of 6±0.2%, with low melting and glass transition temperatures of 36±1.2 and -16±0.8°C, respectively. The polymer exhibited a shear thinning behavior and a mechanical spectrum with a predominant viscous contribution. Its shear bond strength for wood (67±9.4kPa) and glass (65±7.3kPa) suggests it may be used for the development of biobased glues.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - Jan 2016


  • Olive oil deodorizer distillate (OODD)
  • Rheology
  • Shear bond strength


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