Characterization of genomic single nucleotide polymorphism via colorimetric detection using a single gold nanoprobe

Fabio Ferreira Carlos, Orfeu Flores, Goncalo Doria, Pedro Baptista

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Identification of specific nucleic acid sequences mediated by gold nanoparticles derivatized thiol-modified oligonucleotides (Au-nanoprobes) has been proven to be a useful tool in molecular diagnostics. Here, we demonstrate that, on optimization, detection may be simplified via the use of a single Au-nanoprobe to detect a single nucleotide polymorphism (SNP) in homo- or heterozygote condition. We validated this non-cross-linking approach through the analysis of 20 clinical samples using a single specific Au-nanoprobe for an SNP in the FTO (fat mass and obesity-associated) gene against direct DNA sequencing. Sensitivity, specificity, and limit of detection CLOD) were determined, and statistical differences were calculated by one-way analysis of variance (ANOVA) and a post hoc Tukey's test to ascertain whether there were any differences between Au-nanoprobe genotyped groups. For the first time, we show that the use of a single Au-nanoprobe can detect SNP for each genetic status (wild type, heterozygous, or mutant) with high degrees of sensitivity (87.50%) and specificity (91.67%). (c) 2014 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalAnalytical Biochemistry
Volume465
DOIs
Publication statusPublished - 15 Nov 2014

Keywords

  • Gold nanoparticles
  • Nanodiagnostics
  • Non-cross-linking method
  • Point of care
  • FTO gene
  • Genotyping
  • FTO GENE
  • NANOPARTICLES
  • MYCOBACTERIA
  • OBESITY
  • VARIANT
  • DNA

Cite this

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title = "Characterization of genomic single nucleotide polymorphism via colorimetric detection using a single gold nanoprobe",
abstract = "Identification of specific nucleic acid sequences mediated by gold nanoparticles derivatized thiol-modified oligonucleotides (Au-nanoprobes) has been proven to be a useful tool in molecular diagnostics. Here, we demonstrate that, on optimization, detection may be simplified via the use of a single Au-nanoprobe to detect a single nucleotide polymorphism (SNP) in homo- or heterozygote condition. We validated this non-cross-linking approach through the analysis of 20 clinical samples using a single specific Au-nanoprobe for an SNP in the FTO (fat mass and obesity-associated) gene against direct DNA sequencing. Sensitivity, specificity, and limit of detection CLOD) were determined, and statistical differences were calculated by one-way analysis of variance (ANOVA) and a post hoc Tukey's test to ascertain whether there were any differences between Au-nanoprobe genotyped groups. For the first time, we show that the use of a single Au-nanoprobe can detect SNP for each genetic status (wild type, heterozygous, or mutant) with high degrees of sensitivity (87.50{\%}) and specificity (91.67{\%}). (c) 2014 Elsevier Inc. All rights reserved.",
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Characterization of genomic single nucleotide polymorphism via colorimetric detection using a single gold nanoprobe. / Carlos, Fabio Ferreira; Flores, Orfeu; Doria, Goncalo; Baptista, Pedro.

In: Analytical Biochemistry, Vol. 465, 15.11.2014, p. 1-5.

Research output: Contribution to journalArticle

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