@article{5e7ff61c835441b186b219a248a31306,
title = "Characterization of DsrD and its interaction with the DsrAB dissimilatory sulfite reductase",
abstract = "Microbial dissimilatory sulfate reduction is a key process in the global sulfur and carbon cycles in anoxic ecosystems. In this anaerobic respiration, sulfate is phosphorylated and reduced to sulfite, which is further reduced to a DsrC-trisulfide by the dissimilatory sulfite reductase DsrAB. DsrD is a small protein that acts as an allosteric activator of DsrAB, increasing the efficiency of sulfite reduction. Here, we report a detailed study of DsrD and its interaction with DsrAB. Sequence similarity analyses show that there are three groups of DsrD in organisms with a reductive-type DsrAB. The protein regions involved in the DsrD–DsrAB interaction and activity-promoting effect were investigated through in vitro and in silico studies, including mutations of conserved DsrD residues. The results reveal that the conserved β-loop of DsrD is involved in the interaction, contributing to a better understanding of its mechanism of action.",
keywords = "dissimilatory sulfite reductase, DsrAB, DsrD, sulfate-reducing bacteria, sulfur metabolism",
author = "Barbosa, \{Ana C.C.\} and Venceslau, \{Sofia S.\} and Delfim Ferreira and Sinje Neukirchen and Sousa, \{Filipa L.\} and Melo, \{Manuel N.\} and Pereira, \{In{\^e}s A.C.\}",
note = "Funding Information: We thank Jo\textbackslash{}u00E3o Carita from ITQB NOVA for microbial cell growth, and Kazuhiro Umezawa and Manabu Fukui from Hokkaido University for the list of sulfur disproportionator genomes. This work was financially supported by the Funda\textbackslash{}u00E7\textbackslash{}u00E3o para a Ci\textbackslash{}u00EAncia e Tecnologia (Portugal) through Fellowships PD/BD/135488/2018 and COVID/BD/152504/2022 (A.C.C.B.), PD/BD/128204/2016 (D.F.), and CEECIND/04124/2017/CP1428/CT0008 (M.N.M.), Grants PTDC/BIA-MIC/6512/2014 and Grant PTDC/BIA-BQM/29118/2017, Research Unit Molecular, Structural and Cellular Microbiology (MOSTMICRO-ITQB) (UIDB/04612/2020 and UIDP/04612/2020), and Associate Laboratory Life Sciences for a Healthy and Sustainable Future (LS4FUTURE) (LA/P/0087/2020). We acknowledge support from the Wiener Wissenschafts, Forschungs- und Technologiefonds (Austria) through the grant VRG15-007 (FLS). FLS gratefully acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation program (grant agreement 803768). Funding Information: We thank Jo\textbackslash{}u00E3o Carita from ITQB NOVA for microbial cell growth, and Kazuhiro Umezawa and Manabu Fukui from Hokkaido University for the list of sulfur disproportionator genomes. This work was financially supported by the Funda\textbackslash{}u00E7\textbackslash{}u00E3o para a Ci\textbackslash{}u00EAncia e Tecnologia (Portugal) through Fellowships PD/BD/135488/2018 and COVID/BD/152504/2022 (A.C.C.B.), PD/BD/128204/2016 (D.F.), and CEECIND/04124/2017/CP1428/CT0008 (M.N.M.), Grants PTDC/BIA\textbackslash{}u2010MIC/6512/2014 and Grant PTDC/BIA\textbackslash{}u2010BQM/29118/2017, Research Unit Molecular, Structural and Cellular Microbiology (MOSTMICRO\textbackslash{}u2010ITQB) (UIDB/04612/2020 and UIDP/04612/2020), and Associate Laboratory Life Sciences for a Healthy and Sustainable Future (LS4FUTURE) (LA/P/0087/2020). We acknowledge support from the Wiener Wissenschafts, Forschungs\textbackslash{}u2010 und Technologiefonds (Austria) through the grant VRG15\textbackslash{}u2010007 (FLS). FLS gratefully acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation program (grant agreement 803768). Publisher Copyright: {\textcopyright} 2024 The Protein Society.",
year = "2024",
month = dec,
doi = "10.1002/pro.5222",
language = "English",
volume = "33",
journal = "Protein Science",
issn = "0961-8368",
publisher = "Wiley-Blackwell",
number = "12",
}