Characterization of D. desulfuricans (ATCC 27774) [NiFe] hydrogenase EPR and redox properties of the native and the dihydrogen reacted states

Ricardo Franco, Isabel Moura, Jean LeGall, Harry D. Peck, Boi H. Huynh, JoséJ J. G. Moura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Redox intermediates of D. desulfuricans ATCC 27774 [NiFe] hydrogenase were generated under dihydrogen. Detailed redox titrations, coupled to EPR measurements, give access to the mid-point redox potentials of the iron-sulfur centers and of the Nickel-B signal that represents the ready form of the enzyme. The interaction between the dihydrogen molecule and the nickel centre was probed by the observation of an isotopic effect on the EPR signals detected in turnover conditions, by comparison of the H2O/H2 and D2O/D2-reacted samples.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalBBA - Bioenergetics
Volume1144
Issue number3
DOIs
Publication statusPublished - 4 Oct 1993

Keywords

  • EPR
  • Hydrogenase
  • Iron-sulfur center
  • Nickel
  • Redox titration
  • Sulfate-reducing bacterium

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