Characterization of a 7Fe ferredoxin isolated from the marine denitrifier pseudomonas nautica strain 617: Spectroscopic and electrochemical studies

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A 7Fe ferredoxin, isolated from the marine denitrifier Pseudomonas nautica strain 617, was characterized. The NH2-terminal sequence analysis, performed until residue number 56, shows a high similarity with the 7Fe ferredoxins isolated from Azotobacter vinelandii, Pseudomonas putida, and Pseudomonas stutzeri. EPR and NMR spectroscopies identify the presence of both [3Fe-4S] and [4Fe-4S] clusters, with cysteinyl coordination. The electrochemical studies on [Fe-S] clusters show that a fast diffusion-dominated electron transfer, promoted by Mg(II), takes place between the ferredoxin and the glassy carbon electrode. Square wave voltammetry studies gave access to the electrosynthesis of a 4Fe center formed within the [3Fe-4S] core. The [3Fe-4S] cluster exhibited two reduction potentials at -175 and -680 ± 10 mV and the [4Fe-4S] cluster was characterized by an unusually low reduction potential of -715 ± 10 mV, at pH 7.6.

Original languageEnglish
Pages (from-to)524-530
Number of pages7
JournalBiochemical And Biophysical Research Communications
Volume229
Issue number2
DOIs
Publication statusPublished - 13 Dec 1996

Fingerprint Dive into the research topics of 'Characterization of a 7Fe ferredoxin isolated from the marine denitrifier pseudomonas nautica strain 617: Spectroscopic and electrochemical studies'. Together they form a unique fingerprint.

Cite this