Changes in the proteome of Huh7 cells induced by transient expression of hepatitis D virus RNA and antigens

Sérgio Mota, Marta D. Mendes, Deborah Penque, Ana Maria Coelho, Celso Cunha

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Hepatitis delta virus (HDV) infection of human hepatocytes infected with the hepatitis B virus (HBV) is associated with increased liver damage and risk of fulminant disease. Although considerable progress has been made towards the elucidation of the mechanisms of HDV replication and pathogenesis, little is still known about the host factors involved in the different steps of the replication cycle. Here, we made use of a proteomic approach to analyse the global alterations in protein expression that arise in human hepatocytes separately transfected with each of the HDV components. Huh7 cells were transiently transfected with plasmids that code for the small delta antigen (S-HDAg), large delta antigen (L-HDAg), genomic RNA (gRNA), and antigenomic RNA (agRNA), respectively. Total protein extracts were separated by 2-DE and differentially expressed spots were identified by MALDI-TOF followed by database searching. We identified 32 proteins known to be involved in different pathways namely nucleic acid metabolism, protein metabolism, transport, signal transduction, apoptosis, and cell growth. Moreover, the down regulation of hnRNP D, HSP105, and triosephosphate isomerase was further confirmed by Real time PCR.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalJournal of Proteomics
Volume71
Issue number1
DOIs
Publication statusPublished - 30 Apr 2008

Keywords

  • 2-DE
  • Hepatitis D virus
  • Liver
  • Proteome analysis

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

Fingerprint Dive into the research topics of 'Changes in the proteome of Huh7 cells induced by transient expression of hepatitis D virus RNA and antigens'. Together they form a unique fingerprint.

  • Cite this