Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy

Patrícia C. Grácio; Clara Gonçalves-Dias; Filipa Lopes-Coelho; Emília C. Monteiro; Jacinta Serpa; Cláudia L. Da Silva; Sofia A. Pereira

doi:10.1109/ENBENG.2019.8692517

Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy

Patrícia C. Grácio, Clara Gonçalves-Dias, Filipa Lopes-Coelho, Emília C. Monteiro, Jacinta Serpa, Cláudia L. Da Silva, Sofia A. Pereira

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Kidney disease is a worldwide health concern and currently there are no drugs to reverse kidney disease progression. Expanding evidence shows the potential of mesenchymal stromal cell (MSC)-based therapies for kidney tubular disease treatment. This work was aimed to investigate the contribution of the renoprotective enzyme NAT8 for this effect. For that, an in vitro model of differentiation/reepithelization induced by the exposition of tubular cells to TGF-β1 was set up and the effects of conditioned media of MSC on NAT8 during epithelial to mesenchymal transition events were investigated. Results showed that prolonged exposure to TGF-β1 and loss of epithelial markers was associated with a decrease in NAT8 that was reverted by treatment with conditioned media of MSC. We report paracrine effects of human MSC on NAT8 in kidney tubular cells and the relevance of NAT8 in maintaining regenerative potential when TECs undergo dedifferentiation.

Original language	English
Title of host publication	6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
ISBN (Electronic)	9781538685068
DOIs	https://doi.org/10.1109/ENBENG.2019.8692517
Publication status	Published - 15 Apr 2019
Event	6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Lisbon, Portugal Duration: 22 Feb 2019 → 23 Feb 2019

Conference

Conference	6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019
Country/Territory	Portugal
City	Lisbon
Period	22/02/19 → 23/02/19

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/ENBENG.2019.8692517

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Grácio, P. C., Gonçalves-Dias, C., Lopes-Coelho, F., Monteiro, E. C., Serpa, J., Da Silva, C. L., & Pereira, S. A. (2019). Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. In 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings Article 8692517 Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ENBENG.2019.8692517

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title = "Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy",

abstract = "Kidney disease is a worldwide health concern and currently there are no drugs to reverse kidney disease progression. Expanding evidence shows the potential of mesenchymal stromal cell (MSC)-based therapies for kidney tubular disease treatment. This work was aimed to investigate the contribution of the renoprotective enzyme NAT8 for this effect. For that, an in vitro model of differentiation/reepithelization induced by the exposition of tubular cells to TGF-β1 was set up and the effects of conditioned media of MSC on NAT8 during epithelial to mesenchymal transition events were investigated. Results showed that prolonged exposure to TGF-β1 and loss of epithelial markers was associated with a decrease in NAT8 that was reverted by treatment with conditioned media of MSC. We report paracrine effects of human MSC on NAT8 in kidney tubular cells and the relevance of NAT8 in maintaining regenerative potential when TECs undergo dedifferentiation.",

author = "Gr{\'a}cio, {Patr{\'i}cia C.} and Clara Gon{\c c}alves-Dias and Filipa Lopes-Coelho and Monteiro, {Em{\'i}lia C.} and Jacinta Serpa and {Da Silva}, {Cl{\'a}udia L.} and Pereira, {Sofia A.}",

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booktitle = "6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings",

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Grácio, PC, Gonçalves-Dias, C , Lopes-Coelho, F , Monteiro, EC , Serpa, J, Da Silva, CL & Pereira, SA 2019, Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. in 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings., 8692517, Institute of Electrical and Electronics Engineers (IEEE), 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019, Lisbon, Portugal, 22/02/19. https://doi.org/10.1109/ENBENG.2019.8692517

Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. / Grácio, Patrícia C.; Gonçalves-Dias, Clara ; Lopes-Coelho, Filipa et al.
6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings. Institute of Electrical and Electronics Engineers (IEEE), 2019. 8692517.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Changes in N-acetyltransferase 8 in kidney tubular cell

T2 - 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019

AU - Grácio, Patrícia C.

AU - Gonçalves-Dias, Clara

AU - Lopes-Coelho, Filipa

AU - Monteiro, Emília C.

AU - Serpa, Jacinta

AU - Da Silva, Cláudia L.

AU - Pereira, Sofia A.

PY - 2019/4/15

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N2 - Kidney disease is a worldwide health concern and currently there are no drugs to reverse kidney disease progression. Expanding evidence shows the potential of mesenchymal stromal cell (MSC)-based therapies for kidney tubular disease treatment. This work was aimed to investigate the contribution of the renoprotective enzyme NAT8 for this effect. For that, an in vitro model of differentiation/reepithelization induced by the exposition of tubular cells to TGF-β1 was set up and the effects of conditioned media of MSC on NAT8 during epithelial to mesenchymal transition events were investigated. Results showed that prolonged exposure to TGF-β1 and loss of epithelial markers was associated with a decrease in NAT8 that was reverted by treatment with conditioned media of MSC. We report paracrine effects of human MSC on NAT8 in kidney tubular cells and the relevance of NAT8 in maintaining regenerative potential when TECs undergo dedifferentiation.

AB - Kidney disease is a worldwide health concern and currently there are no drugs to reverse kidney disease progression. Expanding evidence shows the potential of mesenchymal stromal cell (MSC)-based therapies for kidney tubular disease treatment. This work was aimed to investigate the contribution of the renoprotective enzyme NAT8 for this effect. For that, an in vitro model of differentiation/reepithelization induced by the exposition of tubular cells to TGF-β1 was set up and the effects of conditioned media of MSC on NAT8 during epithelial to mesenchymal transition events were investigated. Results showed that prolonged exposure to TGF-β1 and loss of epithelial markers was associated with a decrease in NAT8 that was reverted by treatment with conditioned media of MSC. We report paracrine effects of human MSC on NAT8 in kidney tubular cells and the relevance of NAT8 in maintaining regenerative potential when TECs undergo dedifferentiation.

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Grácio PC, Gonçalves-Dias C , Lopes-Coelho F , Monteiro EC , Serpa J, Da Silva CL et al. Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. In 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings. Institute of Electrical and Electronics Engineers (IEEE). 2019. 8692517 doi: 10.1109/ENBENG.2019.8692517

Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy

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