Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess

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Abstract

The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.

Original languageEnglish
Pages (from-to)311-322
Number of pages12
JournalJournal Of Biological Inorganic Chemistry
Volume20
Issue number2
DOIs
Publication statusPublished - 2015
Event2013 Molybdenum and Tungsten Enzymes Conference - Sintra, Portugal
Duration: 16 Jul 201319 Jul 2013

Keywords

  • Hydrogen cycling
  • Microbiologically influenced corrosion
  • Molybdenum
  • Sulfate-reducing bacteria
  • Tungsten

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