Canine neutrophils cooperate with macrophages in the early stages of Leishmania infantum in vitro infection

Maria A Pereira, Graça Alexandre-Pires, Margarida Câmara, Marcos Santos, Catarina Martins, Armanda Rodrigues, Jéssica Adriana, Luiz Felipe D Passero, Isabel Pereira da Fonseca, Gabriela Santos-Gomes

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Leishmania infantum is the aetiological agent of human visceral leishmaniasis and canine leishmaniasis, both systemic and potentially fatal diseases. Polymorphonuclear neutrophils (PMN) are the first cells to phagocyte this parasite at the inoculation site, but macrophages (MØ) are the definitive host cells, ensuring parasite replication. The interaction between dog MØ, PMN and L infantum promastigotes was in vitro investigated. It was observed that promastigotes establish contact with blood monocyte-derived MØ mainly by the tip of the flagellum. These cells, that efficiently bind and internalize parasites, underwent major morphological changes, produced nitric oxide (NO) and released histone H1 in order to inactivate the parasite. Transfer of intracellular parasites from PMN to MØ was confirmed by flow cytometry, using L infantum expressing a green fluorescent protein. The interaction of MØ with L infantum-infected PMN lead to NO production and release of extracellular traps, which may contribute to parasite containment and inactivation. This study highlights for the first time the diversity of cellular and molecular events triggered by the interaction between canine PMN and MØ, which can promote a reduction of parasite burden in the early phase of L infantum infection.

Original languageEnglish
Article numbere12617
Pages (from-to)e12617-e12630
Number of pages13
JournalParasite Immunology
VolumeVol. 41
Issue numbern.º 4
DOIs
Publication statusE-pub ahead of print - 8 Feb 2019

Keywords

  • Canine leishmaniosis
  • Extracellular traps
  • Histone H1
  • Macrophage-neutrophil interaction
  • Parasite transfer

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