Cancer Exosomes as Mediators of Drug Resistance

Maria do Rosário André, Ana Pedro, David Lyden

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Citations (Scopus)

Abstract

In the last decades, several studies demonstrated that the tumor microenvironment is a critical determinant not only of tumor progression and metastasis, but also of resistance to therapy. Exosomes are small membrane vesicles of endocytic origin, which contain mRNAs, DNA fragments, and proteins, and are released by many different cell types, including cancer cells. Mounting evidence has shown that cancer-derived exosomes contribute to the recruitment and reprogramming of constituents associated with the tumor microenvironment. Understanding how exosomes and the tumor microenvironment impact drug resistance will allow novel and better strategies to overcome drug resistance and treat cancer. Here, we describe a technique for exosome purification from cell culture, and fresh and frozen plasma, and further analysis by electron microscopy, NanoSight microscope, and Western blot.

Original languageEnglish
Title of host publicationCancer Exosomes as Mediators of Drug Resistance
EditorsJosé Rueff, António Sebastião Rodrigues
PublisherHumana Press
Pages229-39
Number of pages11
Volume1395
ISBN (Electronic)978-1-4939-3347-1
ISBN (Print)978-1-4939-3345-7
DOIs
Publication statusPublished - 25 Feb 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press Inc
Volume1395
ISSN (Electronic)1064-3745

Keywords

  • Animals
  • Biomarkers, Tumor
  • Blotting, Western
  • Cell Line, Tumor
  • Cryopreservation
  • Drug Resistance, Neoplasm
  • Exosomes
  • Humans
  • Mice
  • Microscopy, Electron
  • Nanoparticles
  • Journal Article

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