Can ultrasonic energy efficiently speed 18O-labeling of proteins?

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Abstract

We report in this work on the robustness of ultrasonic energy as a tool to speed the isotopic labeling of proteins using the 18O-decoupling procedure. The first part of the decoupling procedure, comprising protein denaturation, reduction, alkylation and digestion, is done in 8min under the effects of an ultrasonic field whilst the second part, the isotopic labeling, was assayed with and without the use of ultrasonic energy. Our results clearly demonstrate that the 18O-isotopic labeling in a decoupling procedure cannot be accelerated using an ultrasonic field.

Original languageEnglish
Pages (from-to)4974-4977
Number of pages4
JournalProteomics
Volume9
Issue number21
DOIs
Publication statusPublished - Nov 2009

Keywords

  • O-labeling
  • MALDI-TOF-MS
  • Peptide mapping
  • Sonoreactor
  • Technology
  • Ultrasound

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