TY - JOUR
T1 - Burkholderia thailandensis as a microbial cell factory for the bioconversion of used cooking oil to polyhydroxyalkanoates and rhamnolipids
AU - Kourmentza, C.
AU - Costa, J.
AU - Azevedo, Z.
AU - Servin, C.
AU - Grandfils, C.
AU - Freitas, V. de
AU - Reis, M. A. M.
N1 - info:eu-repo/grantAgreement/EC/FP7/625774/EU#
Dr. Constantina Kourmentza acknowledges the financial support provided from the European Commission through the FP7-PEOPLE-2013-IEF-Marie-Curie Action: Intra-European Fellowships for Career Development (Grant Agreement No: 625774, Acronym: SimPHAsRLs).
PY - 2018/1/1
Y1 - 2018/1/1
N2 -
The present work assessed the feasibility of used cooking oil as a low cost carbon source for rhamnolipid biosurfactant production employing the strain Burkholderia thailandensis. According to the results, B. thailandensis was able to produce rhamnolipids up to 2.2 g/L, with the dominant congener being the di-rhamnolipid Rha-Rha-C
14
-C
14
. Rhamnolipids had the ability to reduce the surface tension to 37.7 mN/m and the interfacial tension against benzene and oleic acid to 4.2 and 1.5 mN/m, while emulsification index against kerosene reached up to 64%. The ability of B. thailandensis to accumulate intracellular biopolymers, in the form of polyhydroxyalkanoates (PHA), was also monitored. Polyhydroxybutyrate (PHB) was accumulated simultaneously and consisted of up to 60% of the cell dry weight. PHB was further characterized in terms of its molecular weight and thermal properties. This is the first study reporting the simultaneous production of polyhydroxyalkanoates and rhamnolipids by the non-pathogen rhamnolipid producer B. thailandensis.
AB -
The present work assessed the feasibility of used cooking oil as a low cost carbon source for rhamnolipid biosurfactant production employing the strain Burkholderia thailandensis. According to the results, B. thailandensis was able to produce rhamnolipids up to 2.2 g/L, with the dominant congener being the di-rhamnolipid Rha-Rha-C
14
-C
14
. Rhamnolipids had the ability to reduce the surface tension to 37.7 mN/m and the interfacial tension against benzene and oleic acid to 4.2 and 1.5 mN/m, while emulsification index against kerosene reached up to 64%. The ability of B. thailandensis to accumulate intracellular biopolymers, in the form of polyhydroxyalkanoates (PHA), was also monitored. Polyhydroxybutyrate (PHB) was accumulated simultaneously and consisted of up to 60% of the cell dry weight. PHB was further characterized in terms of its molecular weight and thermal properties. This is the first study reporting the simultaneous production of polyhydroxyalkanoates and rhamnolipids by the non-pathogen rhamnolipid producer B. thailandensis.
KW - Non-pathogenic bacteria
KW - Polyhydroxyalkanoates
KW - Rhamnolipids
KW - Simultaneous production
KW - Used cooking oil
UR - http://www.scopus.com/inward/record.url?scp=85030683006&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.09.138
DO - 10.1016/j.biortech.2017.09.138
M3 - Article
C2 - 30060419
AN - SCOPUS:85030683006
SN - 0960-8524
VL - 247
SP - 829
EP - 837
JO - Bioresource Technology
JF - Bioresource Technology
ER -