Bronchoalveolar lavage: Quantitative mass spectrometry-based proteomics analysis in lung diseases: Methods in Molecular Biology

Research output: Chapter in Book/Report/Conference proceedingChapter

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Abstract

Bronchoalveolar lavage (BAL) fluid, obtained by a relatively noninvasive procedure, is used as a practice for diagnosis of various lung diseases as source of cells for cytology analysis. The acellular component of BAL potentially can complement and be a key for the establishment of diagnostic or as a prognostic indicator. This chapter discusses the aspects of standardization of BAL sample preparation and processing and its implications on the BAL fluid proteome quantitative analysis by high-throughput mass spectrometry. The detailed conditions for quantitative analysis of BAL proteome in the context of biomarker discovery are introduced. © 2017, Springer Science+Business Media LLC.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages487-494
Number of pages8
Volume1619
ISBN (Print)10643745 (ISSN)
DOIs
Publication statusPublished - 2017

Keywords

  • Biomarkers
  • Bronchoalveolar lavage fluid
  • Diagnosis
  • Lung diseases
  • Mass spectrometry
  • Proteome

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