Bronchoalveolar lavage (BAL) fluid, obtained by a relatively noninvasive procedure, is used as a practice for diagnosis of various lung diseases as source of cells for cytology analysis. The acellular component of BAL potentially can complement and be a key for the establishment of diagnostic or as a prognostic indicator. This chapter discusses the aspects of standardization of BAL sample preparation and processing and its implications on the BAL fluid proteome quantitative analysis by high-throughput mass spectrometry. The detailed conditions for quantitative analysis of BAL proteome in the context of biomarker discovery are introduced. © 2017, Springer Science+Business Media LLC.
- Bronchoalveolar lavage fluid
- Lung diseases
- Mass spectrometry
Carvalho, A. S., & Matthiesen, R. (2017). Bronchoalveolar lavage: Quantitative mass spectrometry-based proteomics analysis in lung diseases: Methods in Molecular Biology. In Methods in Molecular Biology (Vol. 1619, pp. 487-494). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7057-5_34