Breast cancer metabolic cross-talk

Fibroblasts are hubs and breast cancer cells are gatherers of lipids

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cellular components of microenvironment are partners of cancer cells, sharing soluble factors and organic molecules to accomplish tumor energy and biomass demands. We tested the role of fibroblasts in fatty acids metabolism in breast cancer, addressing fatty acid synthase (FASN) expression and activity, the expression of lipids chaperons (FABPs) and transporters (FATPs) and lipids cellular content.We showed that the amount of lipids increased in cancer cells exposed to fibroblasts conditioned media, showing that lipids transfer is crucial in this metabolic cross-talk. Accordingly, it was seen in those cancer cells a concomitant decrease in the expression of FABP2 and FABP3 and an increase in FATP1 expression, whose function is independent of FABPs. The in vivo experiment corroborates the role of CAFs in tumor growth.Our study is one more step toward the understanding of metabolic dynamics between cancer cells and CAFs, disclosing FATP1 as a putative target to disturb the transfer of lipids between CAFs and breast cancer cells.

Original languageEnglish
Pages (from-to)93-106
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume462
Issue numberPart B
DOIs
Publication statusPublished - Feb 2018

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Fibroblasts
Cells
Breast Neoplasms
Lipids
Neoplasms
Tumors
Fatty Acid Synthases
Cellular Microenvironment
Conditioned Culture Medium
Metabolism
Biomass
Fatty Acids
Molecules
Growth
Experiments

Keywords

  • Breast cancer
  • CAFs
  • FASN
  • FATP1
  • Metabolic cross-talk
  • Metabolic fitness
  • Microenvironment

Cite this

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title = "Breast cancer metabolic cross-talk: Fibroblasts are hubs and breast cancer cells are gatherers of lipids",
abstract = "The cellular components of microenvironment are partners of cancer cells, sharing soluble factors and organic molecules to accomplish tumor energy and biomass demands. We tested the role of fibroblasts in fatty acids metabolism in breast cancer, addressing fatty acid synthase (FASN) expression and activity, the expression of lipids chaperons (FABPs) and transporters (FATPs) and lipids cellular content.We showed that the amount of lipids increased in cancer cells exposed to fibroblasts conditioned media, showing that lipids transfer is crucial in this metabolic cross-talk. Accordingly, it was seen in those cancer cells a concomitant decrease in the expression of FABP2 and FABP3 and an increase in FATP1 expression, whose function is independent of FABPs. The in vivo experiment corroborates the role of CAFs in tumor growth.Our study is one more step toward the understanding of metabolic dynamics between cancer cells and CAFs, disclosing FATP1 as a putative target to disturb the transfer of lipids between CAFs and breast cancer cells.",
keywords = "Breast cancer, CAFs, FASN, FATP1, Metabolic cross-talk, Metabolic fitness, Microenvironment",
author = "Filipa Lopes-Coelho and Andr{\'e}, {Maria da Saudade} and Ana Felix and Jacinta Serpa",
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T2 - Fibroblasts are hubs and breast cancer cells are gatherers of lipids

AU - Lopes-Coelho, Filipa

AU - André, Maria da Saudade

AU - Felix, Ana

AU - Serpa, Jacinta

PY - 2018/2

Y1 - 2018/2

N2 - The cellular components of microenvironment are partners of cancer cells, sharing soluble factors and organic molecules to accomplish tumor energy and biomass demands. We tested the role of fibroblasts in fatty acids metabolism in breast cancer, addressing fatty acid synthase (FASN) expression and activity, the expression of lipids chaperons (FABPs) and transporters (FATPs) and lipids cellular content.We showed that the amount of lipids increased in cancer cells exposed to fibroblasts conditioned media, showing that lipids transfer is crucial in this metabolic cross-talk. Accordingly, it was seen in those cancer cells a concomitant decrease in the expression of FABP2 and FABP3 and an increase in FATP1 expression, whose function is independent of FABPs. The in vivo experiment corroborates the role of CAFs in tumor growth.Our study is one more step toward the understanding of metabolic dynamics between cancer cells and CAFs, disclosing FATP1 as a putative target to disturb the transfer of lipids between CAFs and breast cancer cells.

AB - The cellular components of microenvironment are partners of cancer cells, sharing soluble factors and organic molecules to accomplish tumor energy and biomass demands. We tested the role of fibroblasts in fatty acids metabolism in breast cancer, addressing fatty acid synthase (FASN) expression and activity, the expression of lipids chaperons (FABPs) and transporters (FATPs) and lipids cellular content.We showed that the amount of lipids increased in cancer cells exposed to fibroblasts conditioned media, showing that lipids transfer is crucial in this metabolic cross-talk. Accordingly, it was seen in those cancer cells a concomitant decrease in the expression of FABP2 and FABP3 and an increase in FATP1 expression, whose function is independent of FABPs. The in vivo experiment corroborates the role of CAFs in tumor growth.Our study is one more step toward the understanding of metabolic dynamics between cancer cells and CAFs, disclosing FATP1 as a putative target to disturb the transfer of lipids between CAFs and breast cancer cells.

KW - Breast cancer

KW - CAFs

KW - FASN

KW - FATP1

KW - Metabolic cross-talk

KW - Metabolic fitness

KW - Microenvironment

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