Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections

Maïssa Dardouri, Israa M. Aljnadi, Jonas Deuermeier, Catarina Santos, Fabiola Costa, Victor Martin, Maria H. Fernandes, Lídia Gonçalves, Ana Bettencourt, Pedro Sousa Gomes, Isabel A. C. Ribeiro

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
55 Downloads (Pure)

Abstract

In clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.

Original languageEnglish
Article number112679
Number of pages11
JournalColloids and Surfaces B: Biointerfaces
Volume217
DOIs
Publication statusPublished - Sept 2022

Keywords

  • Antibiofilm
  • Antimicrobials
  • Biosurfactants
  • Candida albicans
  • Medical devices
  • PDMS
  • Rhamnolipids
  • Staphylococcus aureus
  • Staphylococcus epidermidis

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