TY - JOUR
T1 - Biomolecular Fishing
T2 - Design, Green Synthesis and Performance of L-Leucine-Molecularly Imprinted Polymers
AU - Furtado, Ana I.
AU - Viveiros, R.
AU - Bonifácio, Vasco D. B.
AU - Melo, André
AU - Casimiro, T.
N1 - info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEQU-EQU%2F32473%2F2017/PT#
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMEC-ONC%2F29327%2F2017/PT#
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FQUI-QIN%2F30649%2F2017/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F150696%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT#
R.V. would like to acknowledge Individual Scientific Employment Stimulus (CEEC-IND), reference 2020.00377.CEECIND from the FCT/MCTES, Portugal.
The Associate Laboratory Research Unit for Green Chemistry–Clean Technologies and Processes–LAQV-REQUIMTE is financed by national funds from FCT/MCTES UID/QUI/50006/2020 and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER–007265).
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Biopurification is a challenging and growing market. Despite great efforts in the past years, current purification strategies still lack specificity, efficiency, and cost-effectiveness. The development of more sustainable functional materials and processes needs to address pressing environmental goals, efficiency, scale-up, and cost. Herein, l-leucine (LEU)-molecularly imprinted polymers (MIPs), LEU-MIPs, are presented as novel biomolecular fishing polymers for affinity sustainable biopurification. Rational design was performed using quantum mechanics calculations and molecular modeling for selecting the most appropriate monomers. LEU-MIPs were synthesized for the first time by two different green approaches, supercritical carbon dioxide (scCO2) technology and mechanochemistry. A significant imprinting factor of 12 and a binding capacity of 27 mg LEU/g polymer were obtained for the LEU-MIP synthesized in scCO2 using 2-vinylpyridine as a functional monomer, while the LEU-MIP using acrylamide as a functional monomer synthesized by mechanochemistry showed an imprinting factor of 1.4 and a binding capacity of 18 mg LEU/g polymer, both systems operating at a low binding concentration (0.5 mg LEU/mL) under physiological conditions. As expected, at a higher concentration (1.5 mg LEU/mL), the binding capacity was considerably increased. Both green technologies show high potential in obtaining ready-to-use, stable, and low-cost polymers with a molecular recognition ability for target biomolecules, being promising materials for biopurification processes.
AB - Biopurification is a challenging and growing market. Despite great efforts in the past years, current purification strategies still lack specificity, efficiency, and cost-effectiveness. The development of more sustainable functional materials and processes needs to address pressing environmental goals, efficiency, scale-up, and cost. Herein, l-leucine (LEU)-molecularly imprinted polymers (MIPs), LEU-MIPs, are presented as novel biomolecular fishing polymers for affinity sustainable biopurification. Rational design was performed using quantum mechanics calculations and molecular modeling for selecting the most appropriate monomers. LEU-MIPs were synthesized for the first time by two different green approaches, supercritical carbon dioxide (scCO2) technology and mechanochemistry. A significant imprinting factor of 12 and a binding capacity of 27 mg LEU/g polymer were obtained for the LEU-MIP synthesized in scCO2 using 2-vinylpyridine as a functional monomer, while the LEU-MIP using acrylamide as a functional monomer synthesized by mechanochemistry showed an imprinting factor of 1.4 and a binding capacity of 18 mg LEU/g polymer, both systems operating at a low binding concentration (0.5 mg LEU/mL) under physiological conditions. As expected, at a higher concentration (1.5 mg LEU/mL), the binding capacity was considerably increased. Both green technologies show high potential in obtaining ready-to-use, stable, and low-cost polymers with a molecular recognition ability for target biomolecules, being promising materials for biopurification processes.
U2 - 10.1021/acsomega.2c05714
DO - 10.1021/acsomega.2c05714
M3 - Article
C2 - 36936318
SN - 2637-6105
VL - 8
SP - 9179
EP - 9186
JO - ACS Applied Polymer Materials
JF - ACS Applied Polymer Materials
IS - 10
ER -