Biomimetic bright optotheranostics for metastasis monitoring and multimodal image-guided breast cancer therapeutics

Rajendra Prasad, Berney Peng, Bárbara B. Mendes, Hailey I. Kilian, Mahadeo Gorain, Huijuan Zhang, Gopal Chandra Kundu, Jun Xia, Jonathan F. Lovell, João Conde

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
14 Downloads (Pure)


Nanoparticle formulations blending optical imaging contrast agents and therapeutics have been a cornerstone of preclinical theranostic applications. However, nanoparticle-based theranostics clinical translation faces challenges on reproducibility, brightness, photostability, biocompatibility, and selective tumor targeting and penetration. In this study, we integrate multimodal imaging and therapeutics within cancer cell-derived nanovesicles, leading to biomimetic bright optotheranostics for monitoring cancer metastasis. Upon NIR light irradiation, the engineered optotheranostics enables deep visualization and precise localization of metastatic lung, liver, and solid breast tumors along with solid tumor ablation. Metastatic cell-derived nanovesicles (∼80 ± 5 nm) are engineered to encapsulate imaging (emissive organic dye and gold nanoparticles) and therapeutic agents (anticancer drug doxorubicin and photothermally active organic indocyanine green dye). Systemic administration of biomimetic bright optotheranostic nanoparticles shows escape from mononuclear phagocytic clearance with (i) rapid tumor accumulation (3 h) and retention (up to 168 h), (ii) real-time monitoring of metastatic lung, liver, and solid breast tumors and (iii) 3-fold image-guided solid tumor reduction. These findings are supported by an improvement of X-ray, fluorescence, and photoacoustic signals while demonstrating a tumor reduction (201 mm3) in comparison with single therapies that includes chemotherapy (134 mm3), photodynamic therapy (72 mm3), and photothermal therapy (88 mm3). The proposed innovative platform opens new avenues to improve cancer diagnosis and treatment outcomes by allowing the monitorization of cancer metastasis, allowing the precise cancer imaging, and delivering synergistic therapeutic agents at the solid tumor site.

Original languageEnglish
Pages (from-to)300-315
Number of pages16
JournalJournal of Controlled Release
Publication statusPublished - Mar 2024


  • Biomimetic
  • Bright optotheranostics
  • Metastasis
  • Multimodal imaging and therapy
  • Solid tumor


Dive into the research topics of 'Biomimetic bright optotheranostics for metastasis monitoring and multimodal image-guided breast cancer therapeutics'. Together they form a unique fingerprint.

Cite this