Biological organomercurial removal from vaccine production wastewaters by a Pseudomonas putida strain

R Fortunato, J. G. Crespo, M. A. Reis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Vaccine production effluents are frequently polluted with thiomersal, a highly toxic organomercurial compound, for which there is presently no remediation technology available. This work proposes a biotechnological process, for the remediation of vaccine production wastewaters, based on the biological degradation of thiomersal to metallic mercury, under aerobic conditions, by a mercury resistant bacterial strain. The kinetics of thiomersal degradation by a pure culture of Pseudomonas putida spi3 strain, in batch culture and using a synthetic wastewater, is presented and discussed. Additionally, a continuous stirred tank reactor (CSTR) fed with the synthetic wastewater was operated, and the bioreactor performance and robustness, when exposed to thiomersal shock loads, was evaluated. Finally, a CSTR for the biological treatment of a real vaccine production effluent was set-up and operated at different dilution rates.
Original languageEnglish
Title of host publicationEUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004
EditorsW. Verstraete
PublisherA A BALKEMA PUBLISHERS
Pages111-114
ISBN (Print)90-5809-653-X
Publication statusPublished - 2004
EventEuropean Symposium on Environmental Biotechnology - Oostende, Belgium
Duration: 25 Apr 200428 Apr 2004

Conference

ConferenceEuropean Symposium on Environmental Biotechnology
CountryBelgium
CityOostende
Period25/04/0428/04/04

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