Biological and molecular characterization of trypanosoma cruzi strains from four states of Brazil

Aline Rimoldi Ribeiro, Luciana Lima, Larissa Aguiar De Almeida, Joana Monteiro, Claudia Jassica Gonçalves Moreno, Juliana Damieli Nascimento, Renato Freitas De Araújo, Fernanda Mello, Luciamare Perinetti Alves Martins, Marcia Aparecida Silva Graminha, Marta Maria Geraldes Teixeira, Marcelo Sousa Silva, Mario Steindel, João Aristeu Da Rosa

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Abstract

Chagas disease affects between six and seven million people. Its etiological agent, Trypanosoma cruzi, is classified into six discrete typing units (DTUs). The biological study of 11 T. cruzi strains presented here included four parameters: growth kinetics, parasitemia curves, rate of macrophage infection, and serology to evaluate IgM, total IgG, IgG1, IgG2a, and IgG3. Sequencing of small subunit of ribosomal RNA (SSU rRNA)was performed and the T. cruzi strains were classified into three DTUs. When their growth in liver infusion tryptose medium was represented in curves, differences among the strains could be noted. The parasitemia profile varied among the strains from the TcI, TcII, and TcIII groups, and the 11 T. cruzi strains produced distinct parasitemia levels in infected BALB/c. The TcI group presented the highest rate of macrophage infection by amastigotes, followed by TcII and TcIII. Reactivity to immunoglobulins was observed in the TcI, TcII, and TcIII; all the animals infected with the different strains of T. cruzi showed anti-T. cruzi antibodies. The molecular study presented here resulted in the classification of the T. cruzi strains into the TcI (Bolivia, T lenti, Tm, SC90); TcII (Famema, SC96, SI8, Y); and TcIII (QMM3, QMM5, SI5) groups. These biological and molecular results from 11 T. cruzi strains clarified the factors involved in the biology of the parasite and its hosts. The collection of triatomine (vector) species, and the study of geographic distribution, as well as biological and molecular characterization of the parasite, will contribute to the reporting and surveillance measures in Brazilian states.

Original languageEnglish
Pages (from-to)453-463
Number of pages11
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume98
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018

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    Ribeiro, A. R., Lima, L., De Almeida, L. A., Monteiro, J., Moreno, C. J. G., Nascimento, J. D., ... Da Rosa, J. A. (2018). Biological and molecular characterization of trypanosoma cruzi strains from four states of Brazil. American Journal of Tropical Medicine and Hygiene, 98(2), 453-463. https://doi.org/10.4269/ajtmh.16-0200