TY - JOUR
T1 - Bioinspired and biomimetic cancer-cell-derived membrane nanovesicles for preclinical tumor-targeted nanotheranostics
AU - Prasad, Rajendra
AU - Mendes, Bárbara B.
AU - Gorain, Mahadeo
AU - Chandra Kundu, Gopal
AU - Gupta, Narendra
AU - Peng, Berney
AU - Aung Win, Eaint Honey
AU - Qing, He
AU - Conde, João
N1 - Funding Information:
J.C. and B.M. acknowledge European Research Council grant agreement 848325 . R.P. would like to thank the Director, Indian Institute of Technology (BHU), Varanasi and the School of Biochemical Engineering, IIT (BHU) for support during preparation of this manuscript. We thank the Trident Diagnostics Center and staff for imaging and laser studies and NCCS, Pune for in vivo facilities. We would like to thank Prof. Rohit Srivastava and Dr. Sumit for their kind support. We extend our thanks to the School of Biotechnology and Kalinga Institute of Medical Sciences, KIIT, Institute of Eminence, Bhubaneswar. We dedicate this article to the memory of the late Prof. Sanjiv Sam Gambhir, a molecular imaging scientist. Figures/schemes were created with BioRender .
Funding Information:
J.C. and B.M. acknowledge European Research Council grant agreement 848325. R.P. would like to thank the Director, Indian Institute of Technology (BHU), Varanasi and the School of Biochemical Engineering, IIT (BHU) for support during preparation of this manuscript. We thank the Trident Diagnostics Center and staff for imaging and laser studies and NCCS, Pune for in vivo facilities. We would like to thank Prof. Rohit Srivastava and Dr. Sumit for their kind support. We extend our thanks to the School of Biotechnology and Kalinga Institute of Medical Sciences, KIIT, Institute of Eminence, Bhubaneswar. We dedicate this article to the memory of the late Prof. Sanjiv Sam Gambhir, a molecular imaging scientist. Figures/schemes were created with BioRender. J.C. and R.P. conceived the idea. M.G. G.C.K. R.P. and J.C. designed the experiments. M.G. G.C.K. R.P. and N.G. performed in vivo imaging and therapeutics studies. B.P. and E.H.A.W. conducted the western blots. B.P. and H.Q. performed the simulation studies. R.P. B.M. and J.C. wrote the paper. All authors contributed to final editing and multiple revisions of the present manuscript. J.C. is a co-founder and shareholder of TargTex S.A. R.P. is part of national and international patents related to lipid, gold, silica, and erythrocyte-based nanoparticles.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/11/15
Y1 - 2023/11/15
N2 - Bioinspired cell-membrane-camouflaged nanohybrids have been proposed to enhance tumor targeting by harnessing their immune escape and self-recognition abilities. In this study, we introduce cancer-cell-derived membrane nanovesicles (CCMVs) integrated with gold nanorods (AuVNRs) in addition to therapeutic and imaging cargos such as doxorubicin and indocyanine green. This approach enhances targeted tumor imaging and enables synergistic chemo-phototherapeutics for solid tumors. CCMVs demonstrate significant tumor penetration and retention, serving as nanotheranostics with accessible surface biomarkers, biomimicking properties, and homologous targeting abilities. By evading uptake by the mononuclear phagocytic system, CCMVs can diffuse into the deep tumor core, leading to precise tumor reduction while preserving the surrounding healthy tissues. Notably, intravenous administration of these theranostic agents ensures biocompatibility, as evidenced by a survival period of approximately two months (up to 63 days) without any observed side effects. Our findings underscore the diagnostic and therapeutic potential of this biomimetic nanotheranostics platform.
AB - Bioinspired cell-membrane-camouflaged nanohybrids have been proposed to enhance tumor targeting by harnessing their immune escape and self-recognition abilities. In this study, we introduce cancer-cell-derived membrane nanovesicles (CCMVs) integrated with gold nanorods (AuVNRs) in addition to therapeutic and imaging cargos such as doxorubicin and indocyanine green. This approach enhances targeted tumor imaging and enables synergistic chemo-phototherapeutics for solid tumors. CCMVs demonstrate significant tumor penetration and retention, serving as nanotheranostics with accessible surface biomarkers, biomimicking properties, and homologous targeting abilities. By evading uptake by the mononuclear phagocytic system, CCMVs can diffuse into the deep tumor core, leading to precise tumor reduction while preserving the surrounding healthy tissues. Notably, intravenous administration of these theranostic agents ensures biocompatibility, as evidenced by a survival period of approximately two months (up to 63 days) without any observed side effects. Our findings underscore the diagnostic and therapeutic potential of this biomimetic nanotheranostics platform.
KW - biomembrane-coated nanoparticles
KW - breast cancer
KW - cancer nanotherapy
KW - homologous targeting
KW - nanovesicles
KW - synergistic therapeutics
KW - theranostics
UR - http://www.scopus.com/inward/record.url?scp=85176302936&partnerID=8YFLogxK
U2 - 10.1016/j.xcrp.2023.101648
DO - 10.1016/j.xcrp.2023.101648
M3 - Article
C2 - 38021344
AN - SCOPUS:85176302936
SN - 2666-3864
VL - 4
JO - Cell Reports Physical Science
JF - Cell Reports Physical Science
IS - 11
M1 - 101648
ER -