TY - JOUR
T1 - Bioelectricity generation using long-term operated biocathode
T2 - RFLP based microbial diversity analysis
AU - Ramanaiah, S. V.
AU - Cordas, Cristina M.
AU - Matias, Sara C.
AU - Reddy, M. Venkateswar
AU - Leitão, Jorge Humberto
AU - Fonseca, Luis P.
N1 - FRH/BPD/33864/2009
UIDB/50006/2020
UIDP/50006/2020
PY - 2021/12
Y1 - 2021/12
N2 - In the present work, power generation and substrate removal efficiencies of long-term operated microbial fuel cells, containing abiotic cathodes and biocathodes, were evaluated for 220 days. Among the two microbial fuel cell (MFC) types, the one containing biocathode showed higher power density (54 mW/m2), current density (122 mA/m2) coulombic efficiency (33%), and substrate removal efficiency (94%) than the abiotic cathode containing MFC. Voltammetric analysis also witnessed higher and sustainable electron discharge for the MFC with biocathode, when compared with the abiotic cathode MFC. Over the tested period, both MFC have shown a cell voltage drop, after 150 and 165, days, for the MFC with biocathode and abiotic cathodes, respectively. Polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) analysis identified 281 clones. Bacteria belonging to Acinetobacter, Acidovorax, Pseudomonas and Burkholderia were observed in the abiotic cathode MFC. Bacteria belonging to Geobacter, Cupriavidus and Acidobacteria were observed in the biocathode MFC. Almost similar types of archaea (Methanosarcinales, Methanolinea, Nitrososphaera and Methanomicrobiales) were observed in both MFCs.
AB - In the present work, power generation and substrate removal efficiencies of long-term operated microbial fuel cells, containing abiotic cathodes and biocathodes, were evaluated for 220 days. Among the two microbial fuel cell (MFC) types, the one containing biocathode showed higher power density (54 mW/m2), current density (122 mA/m2) coulombic efficiency (33%), and substrate removal efficiency (94%) than the abiotic cathode containing MFC. Voltammetric analysis also witnessed higher and sustainable electron discharge for the MFC with biocathode, when compared with the abiotic cathode MFC. Over the tested period, both MFC have shown a cell voltage drop, after 150 and 165, days, for the MFC with biocathode and abiotic cathodes, respectively. Polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) analysis identified 281 clones. Bacteria belonging to Acinetobacter, Acidovorax, Pseudomonas and Burkholderia were observed in the abiotic cathode MFC. Bacteria belonging to Geobacter, Cupriavidus and Acidobacteria were observed in the biocathode MFC. Almost similar types of archaea (Methanosarcinales, Methanolinea, Nitrososphaera and Methanomicrobiales) were observed in both MFCs.
KW - Biocathode
KW - Cyclic voltammetry
KW - Microbial fuel cell
KW - Polymerase chain reaction
KW - restriction fragment length polymorphism (RFLP)
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=85120729573&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2021.e00693
DO - 10.1016/j.btre.2021.e00693
M3 - Article
C2 - 34917493
AN - SCOPUS:85120729573
SN - 2215-017X
VL - 32
SP - 1
EP - 9
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00693
ER -
