Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater

P. Y. Nguyen, Ana F. Silva, Ana C. Reis, Olga C. Nunes, Alexandre M. Rodrigues, João E. Rodrigues, Vitor Vale Cardoso, Maria J. Benoliel, Maria A.M. Reis, Adrian Oehmen, Gilda Carvalho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Achromobacter denitrificans strain PR1, previously found to harbour specific degradation pathways with high sulfamethoxazole (SMX) degradation rates, was bioaugmented into laboratory-scale membrane bioreactors (MBRs) operated under aerobic conditions to treat SMX-containing real domestic wastewater. Different hydraulic retention times (HRTs), which is related to reaction time and loading rates, were considered and found to affect the SMX removal efficiency. The availability of primary substrates was important in both bioaugmented and non-bioaugmented activated sludge (AS) for cometabolism of SMX. High HRT (24 h) resulted in low food to microorganism ratio (F/M) and low SMX removal, due to substrate limitation. Decrease in HRT from 24 h to 12 h, 6 h and finally 4 h led to gradual increases in primary substrates availability, e.g. organic compounds and ammonia, resulted in increased SMX removal efficiency and degradation rate, and is more favorable for high-rate wastewater treatment processes. After inoculation into the MBRs, the bioaugmentation strain was sustained in the reactor for a maximum of 31 days even though a significant decrease in abundance was observed. The bioaugmented MBRs showed enhanced SMX removal, especially under SMX shock loads compared to the control MBRs. The results of this study indicate that re-inoculation is required regularly after a period of time to maintain the removal efficiency of the target compound.

LanguageEnglish
Pages44-55
Number of pages12
JournalScience of the Total Environment
Volume648
Issue number460
DOIs
Publication statusPublished - 15 Jan 2019

Fingerprint

Sulfamethoxazole
Bioreactors
bioreactor
Wastewater
membrane
Membranes
wastewater
Hydraulics
hydraulics
substrate
Degradation
degradation
inoculation
Substrates
Availability
Ports and harbors
oxic conditions
Organic compounds
Wastewater treatment
Microorganisms

Keywords

  • Antibiotics
  • Bioaugmentation
  • Cometabolism
  • Hydraulic retention time
  • Nitrification rate
  • Organic loading rate

Cite this

Nguyen, P. Y. ; Silva, Ana F. ; Reis, Ana C. ; Nunes, Olga C. ; Rodrigues, Alexandre M. ; Rodrigues, João E. ; Cardoso, Vitor Vale ; Benoliel, Maria J. ; Reis, Maria A.M. ; Oehmen, Adrian ; Carvalho, Gilda. / Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater. In: Science of the Total Environment. 2019 ; Vol. 648, No. 460. pp. 44-55.
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abstract = "Achromobacter denitrificans strain PR1, previously found to harbour specific degradation pathways with high sulfamethoxazole (SMX) degradation rates, was bioaugmented into laboratory-scale membrane bioreactors (MBRs) operated under aerobic conditions to treat SMX-containing real domestic wastewater. Different hydraulic retention times (HRTs), which is related to reaction time and loading rates, were considered and found to affect the SMX removal efficiency. The availability of primary substrates was important in both bioaugmented and non-bioaugmented activated sludge (AS) for cometabolism of SMX. High HRT (24 h) resulted in low food to microorganism ratio (F/M) and low SMX removal, due to substrate limitation. Decrease in HRT from 24 h to 12 h, 6 h and finally 4 h led to gradual increases in primary substrates availability, e.g. organic compounds and ammonia, resulted in increased SMX removal efficiency and degradation rate, and is more favorable for high-rate wastewater treatment processes. After inoculation into the MBRs, the bioaugmentation strain was sustained in the reactor for a maximum of 31 days even though a significant decrease in abundance was observed. The bioaugmented MBRs showed enhanced SMX removal, especially under SMX shock loads compared to the control MBRs. The results of this study indicate that re-inoculation is required regularly after a period of time to maintain the removal efficiency of the target compound.",
keywords = "Antibiotics, Bioaugmentation, Cometabolism, Hydraulic retention time, Nitrification rate, Organic loading rate",
author = "Nguyen, {P. Y.} and Silva, {Ana F.} and Reis, {Ana C.} and Nunes, {Olga C.} and Rodrigues, {Alexandre M.} and Rodrigues, {Jo{\~a}o E.} and Cardoso, {Vitor Vale} and Benoliel, {Maria J.} and Reis, {Maria A.M.} and Adrian Oehmen and Gilda Carvalho",
note = "Sem PDF conforme despacho. This research was supported by MERMAID, an Initial Training Network funded by the People Programme (Marie Sklodowska-Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/under REA grant agreement n degrees 607492, and by UCIBIO (FCT/MEC UID/Multi/04378/2013, POCI-01-0145-FEDER-007728). The work was additionally funded by the following projects: (i) POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy - UID/EQU/00511/2013) funded by the European Regional Development Fund (ERDF), through COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI) and by national funds, through FCT; (ii) NORTE-01-0145-FEDER-000005 - LEPABE-2-ECO-INNOVATION, supported by North Portugal Regional Operational Program (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the ERDF. Ana Reis wishes to acknowledge the Portuguese Foundation for Science and Technology (FCT) for her PhD scholarship (grant reference: SFRH/BD/95814/2013). iNOVA4Health Research Unit [grant number LISBOA-01-0145-FEDER-007344], co-funded by Fundacao para a Ciencia e Tecnologia/Ministerio da Ciencia e do Ensino Superior, through national funds, and by FEDER under the PT2020 Partnership Agreement, is acknowledged. Ana Filipa Silva wishes to acknowledge the Portuguese Foundation for Science and Technology (FCT) for her PostDoctoral scholarship (grant reference: SFRH/BPD/97612/2013).",
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Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater. / Nguyen, P. Y.; Silva, Ana F.; Reis, Ana C.; Nunes, Olga C.; Rodrigues, Alexandre M.; Rodrigues, João E.; Cardoso, Vitor Vale; Benoliel, Maria J.; Reis, Maria A.M.; Oehmen, Adrian; Carvalho, Gilda.

In: Science of the Total Environment, Vol. 648, No. 460, 15.01.2019, p. 44-55.

Research output: Contribution to journalArticle

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T1 - Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater

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AU - Silva, Ana F.

AU - Reis, Ana C.

AU - Nunes, Olga C.

AU - Rodrigues, Alexandre M.

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AU - Benoliel, Maria J.

AU - Reis, Maria A.M.

AU - Oehmen, Adrian

AU - Carvalho, Gilda

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PY - 2019/1/15

Y1 - 2019/1/15

N2 - Achromobacter denitrificans strain PR1, previously found to harbour specific degradation pathways with high sulfamethoxazole (SMX) degradation rates, was bioaugmented into laboratory-scale membrane bioreactors (MBRs) operated under aerobic conditions to treat SMX-containing real domestic wastewater. Different hydraulic retention times (HRTs), which is related to reaction time and loading rates, were considered and found to affect the SMX removal efficiency. The availability of primary substrates was important in both bioaugmented and non-bioaugmented activated sludge (AS) for cometabolism of SMX. High HRT (24 h) resulted in low food to microorganism ratio (F/M) and low SMX removal, due to substrate limitation. Decrease in HRT from 24 h to 12 h, 6 h and finally 4 h led to gradual increases in primary substrates availability, e.g. organic compounds and ammonia, resulted in increased SMX removal efficiency and degradation rate, and is more favorable for high-rate wastewater treatment processes. After inoculation into the MBRs, the bioaugmentation strain was sustained in the reactor for a maximum of 31 days even though a significant decrease in abundance was observed. The bioaugmented MBRs showed enhanced SMX removal, especially under SMX shock loads compared to the control MBRs. The results of this study indicate that re-inoculation is required regularly after a period of time to maintain the removal efficiency of the target compound.

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