TY - JOUR
T1 - Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis
AU - Bernacka-Wójcik, Iwona
AU - Lopes, Paulo A.
AU - Vaz, Ana Catarina
AU - Veigas, Bruno Miguel Ribeiro
AU - Wojcik, Pawel Jerzy
AU - Simões, Pedro
AU - Barata, David
AU - Fortunato, Elvira Maria Correia
AU - Baptista, Pedro Miguel Ribeiro Viana
AU - Águas, Hugo Manuel Brito
AU - Martins, Rodrigo Ferrão de Paiva
PY - 2013/10/5
Y1 - 2013/10/5
N2 - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
AB - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
KW - Fibre-optics
KW - DNA
KW - Microfabrication
KW - Nanoparticles
KW - Microfluidics
UR - http://europepmc.org/abstract/med/23660340
U2 - 10.1016/j.bios.2013.03.079
DO - 10.1016/j.bios.2013.03.079
M3 - Article
C2 - 23660340
VL - 48
SP - 87
EP - 93
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
ER -
