Abstract

We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
Original languageUnknown
Pages (from-to)87-93
JournalBiosensors & Bioelectronics
Volume48
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

Cite this

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title = "Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis",
abstract = "We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.",
keywords = "Fibre-optics, DNA, Microfabrication, Nanoparticles, Microfluidics",
author = "Fortunato, {Elvira Maria Correia} and {\'A}guas, {Hugo Manuel Brito} and Martins, {Rodrigo Ferr{\~a}o de Paiva} and Baptista, {Pedro Miguel Ribeiro Viana}",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.bios.2013.03.079",
language = "Unknown",
volume = "48",
pages = "87--93",
journal = "Biosensors & Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Science B.V., Amsterdam.",
number = "NA",

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TY - JOUR

T1 - Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

AU - Fortunato, Elvira Maria Correia

AU - Águas, Hugo Manuel Brito

AU - Martins, Rodrigo Ferrão de Paiva

AU - Baptista, Pedro Miguel Ribeiro Viana

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

AB - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

KW - Fibre-optics

KW - DNA

KW - Microfabrication

KW - Nanoparticles

KW - Microfluidics

U2 - 10.1016/j.bios.2013.03.079

DO - 10.1016/j.bios.2013.03.079

M3 - Article

VL - 48

SP - 87

EP - 93

JO - Biosensors & Bioelectronics

JF - Biosensors & Bioelectronics

SN - 0956-5663

IS - NA

ER -