Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

Elvira Maria Correia Fortunato; Hugo Manuel Brito Águas; Rodrigo Ferrão de Paiva Martins; Pedro Miguel Ribeiro Viana Baptista

doi:10.1016/j.bios.2013.03.079

Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

Elvira Maria Correia Fortunato, Hugo Manuel Brito Águas, Rodrigo Ferrão de Paiva Martins, Pedro Miguel Ribeiro Viana Baptista

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

Original language	Unknown
Pages (from-to)	87-93
Journal	Biosensors & Bioelectronics
Volume	48
Issue number	NA
DOIs	https://doi.org/10.1016/j.bios.2013.03.079
Publication status	Published - 1 Jan 2013

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10.1016/j.bios.2013.03.079

Cite this

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title = "Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis",

abstract = "We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.",

keywords = "Fibre-optics, DNA, Microfabrication, Nanoparticles, Microfluidics",

author = "Fortunato, {Elvira Maria Correia} and {\'A}guas, {Hugo Manuel Brito} and Martins, {Rodrigo Ferr{\~a}o de Paiva} and Baptista, {Pedro Miguel Ribeiro Viana}",

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doi = "10.1016/j.bios.2013.03.079",

language = "Unknown",

volume = "48",

pages = "87--93",

journal = "Biosensors & Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Science B.V., Amsterdam.",

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Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis. / Fortunato, Elvira Maria Correia ; Águas, Hugo Manuel Brito ; Martins, Rodrigo Ferrão de Paiva ; Baptista, Pedro Miguel Ribeiro Viana.

In: Biosensors & Bioelectronics, Vol. 48, No. NA, 01.01.2013, p. 87-93.

Research output: Contribution to journal › Article › peer-review

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T1 - Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

AU - Fortunato, Elvira Maria Correia

AU - Águas, Hugo Manuel Brito

AU - Martins, Rodrigo Ferrão de Paiva

AU - Baptista, Pedro Miguel Ribeiro Viana

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

AB - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

KW - Fibre-optics

KW - DNA

KW - Microfabrication

KW - Nanoparticles

KW - Microfluidics

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DO - 10.1016/j.bios.2013.03.079

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JO - Biosensors & Bioelectronics

JF - Biosensors & Bioelectronics

SN - 0956-5663

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