Abstract
| Original language | Unknown |
|---|---|
| Pages (from-to) | 87-93 |
| Journal | Biosensors & Bioelectronics |
| Volume | 48 |
| Issue number | NA |
| DOIs | |
| Publication status | Published - 1 Jan 2013 |
Cite this
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Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis. / Fortunato, Elvira Maria Correia; Águas, Hugo Manuel Brito; Martins, Rodrigo Ferrão de Paiva; Baptista, Pedro Miguel Ribeiro Viana.
In: Biosensors & Bioelectronics, Vol. 48, No. NA, 01.01.2013, p. 87-93.Research output: Contribution to journal › Article
TY - JOUR
T1 - Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis
AU - Fortunato, Elvira Maria Correia
AU - Águas, Hugo Manuel Brito
AU - Martins, Rodrigo Ferrão de Paiva
AU - Baptista, Pedro Miguel Ribeiro Viana
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
AB - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
KW - Fibre-optics
KW - DNA
KW - Microfabrication
KW - Nanoparticles
KW - Microfluidics
U2 - 10.1016/j.bios.2013.03.079
DO - 10.1016/j.bios.2013.03.079
M3 - Article
VL - 48
SP - 87
EP - 93
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
IS - NA
ER -