Abstract

We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
Original languageUnknown
Pages (from-to)87-93
JournalBiosensors & Bioelectronics
Volume48
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

    Cite this

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    title = "Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis",
    abstract = "We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.",
    keywords = "Fibre-optics, DNA, Microfabrication, Nanoparticles, Microfluidics",
    author = "Fortunato, {Elvira Maria Correia} and {\'A}guas, {Hugo Manuel Brito} and Martins, {Rodrigo Ferr{\~a}o de Paiva} and Baptista, {Pedro Miguel Ribeiro Viana}",
    year = "2013",
    month = "1",
    day = "1",
    doi = "10.1016/j.bios.2013.03.079",
    language = "Unknown",
    volume = "48",
    pages = "87--93",
    journal = "Biosensors & Bioelectronics",
    issn = "0956-5663",
    publisher = "Elsevier Science B.V., Amsterdam.",
    number = "NA",

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    TY - JOUR

    T1 - Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

    AU - Fortunato, Elvira Maria Correia

    AU - Águas, Hugo Manuel Brito

    AU - Martins, Rodrigo Ferrão de Paiva

    AU - Baptista, Pedro Miguel Ribeiro Viana

    PY - 2013/1/1

    Y1 - 2013/1/1

    N2 - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

    AB - We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

    KW - Fibre-optics

    KW - DNA

    KW - Microfabrication

    KW - Nanoparticles

    KW - Microfluidics

    U2 - 10.1016/j.bios.2013.03.079

    DO - 10.1016/j.bios.2013.03.079

    M3 - Article

    VL - 48

    SP - 87

    EP - 93

    JO - Biosensors & Bioelectronics

    JF - Biosensors & Bioelectronics

    SN - 0956-5663

    IS - NA

    ER -