Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

Elvira Maria Correia Fortunato; Hugo Manuel Brito Águas; Rodrigo Ferrão de Paiva Martins; Pedro Miguel Ribeiro Viana Baptista

doi:10.1016/j.bios.2013.03.079

Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis

Elvira Maria Correia Fortunato, Hugo Manuel Brito Águas, Rodrigo Ferrão de Paiva Martins, Pedro Miguel Ribeiro Viana Baptista

Research output: Contribution to journal › Article

29 Citations (Scopus)

Abstract

We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

Original language	Unknown
Pages (from-to)	87-93
Journal	Biosensors & Bioelectronics
Volume	48
Issue number	NA
DOIs	https://doi.org/10.1016/j.bios.2013.03.079
Publication status	Published - 1 Jan 2013

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10.1016/j.bios.2013.03.079

Cite this

Fortunato, E. M. C., Águas, H. M. B., Martins, R. F. D. P., & Baptista, P. M. R. V. (2013). Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis. Biosensors & Bioelectronics, 48(NA), 87-93. https://doi.org/10.1016/j.bios.2013.03.079

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AU - Baptista, Pedro Miguel Ribeiro Viana

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