Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors

A. R. Nunes, E. C. Monteiro, S. M. Johnson, E. B. Gauda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Citations (Scopus)

Abstract

Peripheral arterial chemoreceptors in the carotid body (CB) are modulated by pH/CO2. Soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate ions (HCO3 -). Because CO2/HCO 3 - mediates depolarization in chemoreceptors, we hypothesized that sAC mRNA would be expressed in the CB, and its expression and function would be regulated by CO2/HCO3 -. Sprague-Dawley rats at postnatal days 16-17 were used to compare sAC mRNA gene expression between CB and non-chemosensitive tissues: superior cervical (SCG), petrosal (PG) and nodose ganglia (NG) by quantitative real time-PCR. Rat sAC gene expression was standardized to the expression of GAPDH (housekeeping gene) and the data were analyzed with the Pfaffl method. Gene and protein expression, and sAC regulation in the testis was used as a positive control. To determine the regulation of sAC mRNA expression and activity, all tissues were exposed to increasing concentrations of bicarbonate (0, 24, 44 mM, titrated with CO 2 and maintained a constant pH of 7.40). RESULTS: sAC mRNA expression was between 2-11% of CB expression in the SCG, PG and NG. Furthermore, only in the CB did HCO3 - upregulate sAC gene expression and increase cAMP levels. Conclusion: sAC mRNA and protein expression is present in peripheral arterial chemoreceptors and non-chemoreceptors. In the CB, CO 2/HCO3 - not only activated sAC but also regulated its expression, suggesting that sAC may be involved in the regulation of cAMP levels in response to hyper/hypocapnia.

Original languageEnglish
Title of host publicationArterial Chemoreceptors
Pages235-241
Number of pages7
DOIs
Publication statusPublished - Dec 2009

Publication series

NameAdvances in Experimental Medicine and Biology
Volume648
ISSN (Print)0065-2598

Fingerprint

Bicarbonates
Adenylyl Cyclases
Carotid Body
Messenger RNA
Gene expression
Nodose Ganglion
Gene Expression
Carbon Monoxide
Rats
Tissue
Hypocapnia
Essential Genes
Depolarization
Sprague Dawley Rats
Testis
Real-Time Polymerase Chain Reaction
Proteins
Up-Regulation
Genes

Keywords

  • cAMP
  • Carotid body (CB)
  • CO/HCO
  • Peripheral arterial chemoreceptors
  • qRT-PCR
  • Soluble adenylyl cyclase (sAC)

Cite this

Nunes, A. R., Monteiro, E. C., Johnson, S. M., & Gauda, E. B. (2009). Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors. In Arterial Chemoreceptors (pp. 235-241). (Advances in Experimental Medicine and Biology; Vol. 648). https://doi.org/10.1007/978-90-481-2259-2_27
Nunes, A. R. ; Monteiro, E. C. ; Johnson, S. M. ; Gauda, E. B. / Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors. Arterial Chemoreceptors. 2009. pp. 235-241 (Advances in Experimental Medicine and Biology).
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title = "Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors",
abstract = "Peripheral arterial chemoreceptors in the carotid body (CB) are modulated by pH/CO2. Soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate ions (HCO3 -). Because CO2/HCO 3 - mediates depolarization in chemoreceptors, we hypothesized that sAC mRNA would be expressed in the CB, and its expression and function would be regulated by CO2/HCO3 -. Sprague-Dawley rats at postnatal days 16-17 were used to compare sAC mRNA gene expression between CB and non-chemosensitive tissues: superior cervical (SCG), petrosal (PG) and nodose ganglia (NG) by quantitative real time-PCR. Rat sAC gene expression was standardized to the expression of GAPDH (housekeeping gene) and the data were analyzed with the Pfaffl method. Gene and protein expression, and sAC regulation in the testis was used as a positive control. To determine the regulation of sAC mRNA expression and activity, all tissues were exposed to increasing concentrations of bicarbonate (0, 24, 44 mM, titrated with CO 2 and maintained a constant pH of 7.40). RESULTS: sAC mRNA expression was between 2-11{\%} of CB expression in the SCG, PG and NG. Furthermore, only in the CB did HCO3 - upregulate sAC gene expression and increase cAMP levels. Conclusion: sAC mRNA and protein expression is present in peripheral arterial chemoreceptors and non-chemoreceptors. In the CB, CO 2/HCO3 - not only activated sAC but also regulated its expression, suggesting that sAC may be involved in the regulation of cAMP levels in response to hyper/hypocapnia.",
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Nunes, AR, Monteiro, EC, Johnson, SM & Gauda, EB 2009, Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors. in Arterial Chemoreceptors. Advances in Experimental Medicine and Biology, vol. 648, pp. 235-241. https://doi.org/10.1007/978-90-481-2259-2_27

Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors. / Nunes, A. R.; Monteiro, E. C.; Johnson, S. M.; Gauda, E. B.

Arterial Chemoreceptors. 2009. p. 235-241 (Advances in Experimental Medicine and Biology; Vol. 648).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors

AU - Nunes, A. R.

AU - Monteiro, E. C.

AU - Johnson, S. M.

AU - Gauda, E. B.

PY - 2009/12

Y1 - 2009/12

N2 - Peripheral arterial chemoreceptors in the carotid body (CB) are modulated by pH/CO2. Soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate ions (HCO3 -). Because CO2/HCO 3 - mediates depolarization in chemoreceptors, we hypothesized that sAC mRNA would be expressed in the CB, and its expression and function would be regulated by CO2/HCO3 -. Sprague-Dawley rats at postnatal days 16-17 were used to compare sAC mRNA gene expression between CB and non-chemosensitive tissues: superior cervical (SCG), petrosal (PG) and nodose ganglia (NG) by quantitative real time-PCR. Rat sAC gene expression was standardized to the expression of GAPDH (housekeeping gene) and the data were analyzed with the Pfaffl method. Gene and protein expression, and sAC regulation in the testis was used as a positive control. To determine the regulation of sAC mRNA expression and activity, all tissues were exposed to increasing concentrations of bicarbonate (0, 24, 44 mM, titrated with CO 2 and maintained a constant pH of 7.40). RESULTS: sAC mRNA expression was between 2-11% of CB expression in the SCG, PG and NG. Furthermore, only in the CB did HCO3 - upregulate sAC gene expression and increase cAMP levels. Conclusion: sAC mRNA and protein expression is present in peripheral arterial chemoreceptors and non-chemoreceptors. In the CB, CO 2/HCO3 - not only activated sAC but also regulated its expression, suggesting that sAC may be involved in the regulation of cAMP levels in response to hyper/hypocapnia.

AB - Peripheral arterial chemoreceptors in the carotid body (CB) are modulated by pH/CO2. Soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate ions (HCO3 -). Because CO2/HCO 3 - mediates depolarization in chemoreceptors, we hypothesized that sAC mRNA would be expressed in the CB, and its expression and function would be regulated by CO2/HCO3 -. Sprague-Dawley rats at postnatal days 16-17 were used to compare sAC mRNA gene expression between CB and non-chemosensitive tissues: superior cervical (SCG), petrosal (PG) and nodose ganglia (NG) by quantitative real time-PCR. Rat sAC gene expression was standardized to the expression of GAPDH (housekeeping gene) and the data were analyzed with the Pfaffl method. Gene and protein expression, and sAC regulation in the testis was used as a positive control. To determine the regulation of sAC mRNA expression and activity, all tissues were exposed to increasing concentrations of bicarbonate (0, 24, 44 mM, titrated with CO 2 and maintained a constant pH of 7.40). RESULTS: sAC mRNA expression was between 2-11% of CB expression in the SCG, PG and NG. Furthermore, only in the CB did HCO3 - upregulate sAC gene expression and increase cAMP levels. Conclusion: sAC mRNA and protein expression is present in peripheral arterial chemoreceptors and non-chemoreceptors. In the CB, CO 2/HCO3 - not only activated sAC but also regulated its expression, suggesting that sAC may be involved in the regulation of cAMP levels in response to hyper/hypocapnia.

KW - cAMP

KW - Carotid body (CB)

KW - CO/HCO

KW - Peripheral arterial chemoreceptors

KW - qRT-PCR

KW - Soluble adenylyl cyclase (sAC)

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M3 - Conference contribution

SN - 9789048122585

T3 - Advances in Experimental Medicine and Biology

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EP - 241

BT - Arterial Chemoreceptors

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Nunes AR, Monteiro EC, Johnson SM, Gauda EB. Bicarbonate-regulated soluble adenylyl cyclase (sAC) mRNA expression and activity in peripheral chemoreceptors. In Arterial Chemoreceptors. 2009. p. 235-241. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-90-481-2259-2_27