Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen

Marinela Contreras, Juan A. Moreno-Cid, Ana Domingos, Mario Canales, Iratxe Díez-Delgado, José M. Pérez de la Lastra, Emilio Sánchez, Octávio Merino, Rigoberto López Zavala, Nieves Ayllón, Mariana Boadella, Margarita Villar, Christian Gortázar, José de la Fuente

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-. Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.

Original languageEnglish
Pages (from-to)820-828
Number of pages9
JournalTicks and Tick-borne Diseases
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Sep 2015

Keywords

  • Chimera
  • Endotoxin
  • Immunology
  • MSP1a
  • Subolesin
  • Tick
  • Vaccine

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