Backbone, side chain and heme resonance assignments of the triheme cytochrome PpcD from Geobacter sulfurreducens

Joana M. Dantas, Carlos A. Salgueiro, Marta Bruix

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gene knock-out studies on Geobacter sulfurreducens (Gs) cells showed that the periplasmic triheme cytochrome PpcD is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI) oxides. More recently, it was also shown that the gene encoding for PpcD has higher transcript abundance when Gs cells utilize graphite electrodes as sole electron donors to reduce fumarate. This sets PpcD as the first multiheme cytochrome to be involved in Gs respiratory pathways that bridge the electron transfer between the cytoplasm and cell exterior in both directions. Nowadays, extracellular electron transfer (EET) processes are explored for several biotechnological applications, which include bioremediation, bioenergy and biofuel production. Therefore, the structural characterization of PpcD is a fundamental step to understand the mechanisms underlying EET. However, compared to non-heme proteins, the presence of numerous proton-containing groups in the redox centers presents additional challenges for protein signal assignment and structure calculation. Here, we report the complete assignment of the heme proton signals together with 1H, 13C and 15N backbone and side chain assignments of the reduced form of PpcD.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalBiomolecular Nmr Assignments
Volume9
Issue number1
DOIs
Publication statusPublished - 2015

Keywords

  • Geobacter sulfurreducens
  • Multiheme
  • NMR
  • PpcD

Fingerprint Dive into the research topics of 'Backbone, side chain and heme resonance assignments of the triheme cytochrome PpcD from Geobacter sulfurreducens'. Together they form a unique fingerprint.

  • Cite this