Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens

Research output: Contribution to journalArticle

Abstract

Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalBiomolecular Nmr Assignments
Volume13
Issue number2
DOIs
Publication statusPublished - 15 Oct 2019

Fingerprint

Geobacter
Cytochromes
Electrons
Environmental technology
Gastrin-Secreting Cells
Gene Knockout Techniques
Proteins
Gene encoding
Molecular interactions
Gene Deletion
Biofilms
Transcription
Heme
Metabolism
Microorganisms
Oxidation-Reduction
Hydrogen
Electrodes
Genes
Technology

Keywords

  • Cytochrome
  • Geobacter sulfurreducens
  • Microbial electrosynthesis
  • NMR
  • PccH

Cite this

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title = "Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens",
abstract = "Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.",
keywords = "Cytochrome, Geobacter sulfurreducens, Microbial electrosynthesis, NMR, PccH",
author = "Teixeira, {Liliana R.} and Portela, {Pilar C.} and Leonor Morgado and David Pantoja-Uceda and Marta Bruix and Salgueiro, {Carlos A.}",
note = "info:eu-repo/grantAgreement/FCT/5876/147412/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) Grants: PTDC/BIA-BQM/31981/2017 (to CAS) and scholarship Grant No. PD/BD/114445/2016 (to LRT) through Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012, UID/FIS/00068/2013 (CEFITEC). co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We acknowledge the use of the {"}Manuel Rico{"} NMR laboratory, LMR (CSIC), a Spanish large-scale national NMR facility ICTS R-LRB.",
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AU - Teixeira, Liliana R.

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AU - Morgado, Leonor

AU - Pantoja-Uceda, David

AU - Bruix, Marta

AU - Salgueiro, Carlos A.

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N2 - Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.

AB - Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.

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