Abstract
Os animais podem constituir fonte de infecção demicrosporídeos para o Homem. Pretendeu-se com o presente trabalho, identificar microsporídeos patogénicos para o Homem,em várias espécies de aves e cães.Durante o ano de 2004, foram analisadas amostras de fezes de 132 animais, incluindo 44 pombos domésticos, 38 aves exóticase 50 cães errantes. Os métodos de diagnóstico laboratorial utilizados foram a coloração pelo Gram-chromotrope, a imuno-fluorescência indirecta (IFI) com o anticorpo monoclonal (AcM)3B6 e a técnica de reacção em cadeia pela polimerase (PCR). Neste estudo, a percentagem de amostras positivas para microsporídeos foi de 13% (17/132). As amostras positivas foram detectadas em cinco pombos domésticos, três papagaios cinzentos, duas caturras, um periquito, um diamante estrela, dois tentilhões japoneses e em três cães. Encephalitozoon hellem e Enterocytozoon bieneusi foram as espécies identificadas. Estes resultados obtidos constituem o primeiro registo de infecção por E. hellem e E. bieneusi em aves, em Portugal. Especula-se sobre os possíveis modos de transmissão de microsporídeos, das aves para o Homem. Assim, os pombos domésticos e algumas espécies de aves exóticas e, por outro lado, os pombos domésticos e os cães, constituem fontes potenciais de infecção, respectivamente por E. hellem e por E. bieneusi, para o Homem.
Animals could be a source of infection of microporidia to humans. The present work intends to determine the occurrence of human pathogenic microsporidia in birds and dogs.During the year 2004, a total of 132 animals, including 44 free-living pigeons, 38 exotic birds, and 50 stray dogs were examined. The detection of microsporidia was made by Gram-Chromotrope stain, by immunofluorescence staining with fluores-cein-labelled monoclonal antibody (MAb) 3B6, and by polymerase chain reaction (PCR). Of the 132 animals examined, 13% (17/132) were positive formicrosporidia (five free-living pigeons, three African grey parrots, two cockatiels, one budgerigar, one star finch, two Ben-galese finches, and three stray dogs). The microsporidian speciesidentified were Encephalitozoon hellem and Enterocytozoon bieneusi. These results represent the first report of E. hellem and E. bieneusi in birds, in Portugal. Possible modes of transmissionof microsporidia from birds to humans are discussed. It was concluded that free-living pigeons and some exotic bird species, and free-living pigeons and dogs, are respectively potential sources ofE. hellem and E. bieneusi to man.
Animals could be a source of infection of microporidia to humans. The present work intends to determine the occurrence of human pathogenic microsporidia in birds and dogs.During the year 2004, a total of 132 animals, including 44 free-living pigeons, 38 exotic birds, and 50 stray dogs were examined. The detection of microsporidia was made by Gram-Chromotrope stain, by immunofluorescence staining with fluores-cein-labelled monoclonal antibody (MAb) 3B6, and by polymerase chain reaction (PCR). Of the 132 animals examined, 13% (17/132) were positive formicrosporidia (five free-living pigeons, three African grey parrots, two cockatiels, one budgerigar, one star finch, two Ben-galese finches, and three stray dogs). The microsporidian speciesidentified were Encephalitozoon hellem and Enterocytozoon bieneusi. These results represent the first report of E. hellem and E. bieneusi in birds, in Portugal. Possible modes of transmissionof microsporidia from birds to humans are discussed. It was concluded that free-living pigeons and some exotic bird species, and free-living pigeons and dogs, are respectively potential sources ofE. hellem and E. bieneusi to man.
Translated title of the contribution | Birds and dogs as potencial sources of zoonotic infections by microsporidia |
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Original language | Portuguese |
Pages (from-to) | 69–75 |
Number of pages | 7 |
Journal | Revista Portuguesa de Ciências Veterinárias |
Volume | Vol. 101 |
Issue number | n.º 557-558 |
Publication status | Published - Jan 2006 |
Keywords
- Microsporidiosis
- Zoonosis
- Enterocytozoon
- Encephalitozoon