AuAg-alloy-nanoprobes for specific nucleic acid detection

G. Doria; J. T. Dias; M. Larguinho; E. Pereira; R. Franco; P. Baptista

AuAg-alloy-nanoprobes for specific nucleic acid detection

G. Doria, J. T. Dias, M. Larguinho, E. Pereira, R. Franco, P. Baptista

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.

Original language	English
Title of host publication	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Publisher	Nano Science and Technology Institute
Pages	62-65
Number of pages	4
Volume	3
ISBN (Print)	9781439834152
Publication status	Published - 1 Jan 2010
Event	2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, United States Duration: 21 Jun 2010 → 25 Jun 2010

Conference

Conference	2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/Territory	United States
City	Anaheim
Period	21/06/10 → 25/06/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Diagnostic
Gold
Nanoparticles
Nucleic acid
Silver

Cite this

Doria, G., Dias, J. T., Larguinho, M., Pereira, E., Franco, R., & Baptista, P. (2010). AuAg-alloy-nanoprobes for specific nucleic acid detection. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (Vol. 3, pp. 62-65). Nano Science and Technology Institute.

@inproceedings{2378afa5afa949e0bfbf65598c159350,

title = "AuAg-alloy-nanoprobes for specific nucleic acid detection",

abstract = "The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.",

keywords = "Diagnostic, Gold, Nanoparticles, Nucleic acid, Silver",

author = "G. Doria and Dias, \{J. T.\} and M. Larguinho and E. Pereira and R. Franco and P. Baptista",

year = "2010",

month = jan,

day = "1",

language = "English",

isbn = "9781439834152",

volume = "3",

pages = "62--65",

booktitle = "Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010",

publisher = "Nano Science and Technology Institute",

note = "2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 ; Conference date: 21-06-2010 Through 25-06-2010",

}

Doria, G , Dias, JT , Larguinho, M, Pereira, E, Franco, R & Baptista, P 2010, AuAg-alloy-nanoprobes for specific nucleic acid detection. in Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. vol. 3, Nano Science and Technology Institute, pp. 62-65, 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010, Anaheim, California, United States, 21/06/10.

AuAg-alloy-nanoprobes for specific nucleic acid detection. / Doria, G.; Dias, J. T.; Larguinho, M. et al.
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. Vol. 3 Nano Science and Technology Institute, 2010. p. 62-65.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - AuAg-alloy-nanoprobes for specific nucleic acid detection

AU - Doria, G.

AU - Dias, J. T.

AU - Larguinho, M.

AU - Pereira, E.

AU - Franco, R.

AU - Baptista, P.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.

AB - The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.

KW - Diagnostic

KW - Gold

KW - Nanoparticles

KW - Nucleic acid

KW - Silver

UR - https://www.scopus.com/pages/publications/78049444202

M3 - Conference contribution

AN - SCOPUS:78049444202

SN - 9781439834152

VL - 3

SP - 62

EP - 65

BT - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010

PB - Nano Science and Technology Institute

T2 - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010

Y2 - 21 June 2010 through 25 June 2010

ER -

AuAg-alloy-nanoprobes for specific nucleic acid detection

Abstract

Conference

UN SDGs

Keywords

Other files and links

Fingerprint

Cite this