1 Citation (Scopus)

Abstract

The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.

Original languageEnglish
Title of host publicationNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
PublisherNano Science and Technology Institute
Pages62-65
Number of pages4
Volume3
ISBN (Print)9781439834152
Publication statusPublished - 1 Jan 2010
Event2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, United States
Duration: 21 Jun 201025 Jun 2010

Conference

Conference2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim
Period21/06/1025/06/10

Keywords

  • Diagnostic
  • Gold
  • Nanoparticles
  • Nucleic acid
  • Silver

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  • Cite this

    Doria, G., Dias, J. T., Larguinho, M., Pereira, E., Franco, R., & Baptista, P. (2010). AuAg-alloy-nanoprobes for specific nucleic acid detection. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (Vol. 3, pp. 62-65). Nano Science and Technology Institute.