Abstract
The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.
| Original language | English |
|---|---|
| Title of host publication | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
| Publisher | Nano Science and Technology Institute |
| Pages | 62-65 |
| Number of pages | 4 |
| Volume | 3 |
| ISBN (Print) | 9781439834152 |
| Publication status | Published - 1 Jan 2010 |
| Event | 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, United States Duration: 21 Jun 2010 → 25 Jun 2010 |
Conference
| Conference | 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
|---|---|
| Country/Territory | United States |
| City | Anaheim |
| Period | 21/06/10 → 25/06/10 |
Keywords
- Diagnostic
- Gold
- Nanoparticles
- Nucleic acid
- Silver
