AuAg-alloy-nanoprobes for specific nucleic acid detection

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)


The derivatization of gold-silver alloy nanoparticles with thiol-ssDNA oligonucleotides (AuAg-alloy-nanoprobes) and their use in nucleic acid detection is presented. A non-cross-linking method has been previously developed by our group using gold nanoparticles, which is based on the colorimetric comparison of solutions before and after salt-induced nanoprobe aggregation. Only the presence of a complementary target stabilizes the nanoprobe, preventing aggregation and colorimetric change after salt addition. Through this approach, the AuAg-alloy-nanoprobes allowed to specifically detect a sequence derived from the RNA polymerase β-subunit gene of Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, with a 2.5-fold enhanced sensitivity (0.3 μg of total DNA) when compared to their gold counterparts.

Original languageEnglish
Title of host publicationNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
PublisherNano Science and Technology Institute
Number of pages4
ISBN (Print)9781439834152
Publication statusPublished - 1 Jan 2010
Event2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, United States
Duration: 21 Jun 201025 Jun 2010


Conference2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/TerritoryUnited States


  • Diagnostic
  • Gold
  • Nanoparticles
  • Nucleic acid
  • Silver


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