TY - JOUR
T1 - Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
AU - Araújo, Marília Ladeira de
AU - Gomes, Bruno Costa
AU - Devóz, Paula Pícoli
AU - Duarte, Nathália de Assis Aguilar
AU - Ribeiro, Diego Luis
AU - Araújo, Adriana Ladeira de
AU - Batista, Bruno Lemos
AU - Antunes, Lusânia Maria Greggi
AU - Barbosa, Fernando
AU - Rodrigues, António Sebastião
AU - Rueff, José
AU - Barcelos, Gustavo Rafael Mazzaron
N1 - This study was supported by the grants #2013/06033- 8, #2016/24520-1 and #2018/24643-1 (São Paulo Research Foundation, FAPESP, Brazil), by the National Council for Scientific and Technological Development (Brazil) and by grant UID-BIM-00009-2020 (Foundation for Science and Technology, FCT, Portugal).
PY - 2021/2/17
Y1 - 2021/2/17
N2 - Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.
AB - Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.
KW - epigenetics
KW - lead
KW - miRNA
KW - ncRNA
KW - occupational exposure
KW - toxicity
U2 - 10.3389/fgene.2021.620744
DO - 10.3389/fgene.2021.620744
M3 - Article
C2 - 33679885
SN - 1664-8021
VL - 12
SP - 49
JO - Frontiers in Genetics
JF - Frontiers in Genetics
M1 - 620744
ER -