Associating Wound-Related Changes in the Apoplast Proteome of Medicago with Early Steps in the RIDS Signal-Transduction Pathway

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Early wound-related changes in the leaf apoplast proteome of Medicago, truncatula have been characterized by 2-DE and MALDI-TOF/TOF and the differential expression of 28/110 extracellular proteins could be reproducibly observed 6 h after wounding. Wounding induced an initial (0-30 min) burst of O-2(-), followed by a later (3-6 h) production Of O-2(-) and H2O2. The infiltration of 5 mu M DPI <= 3 min after wounding inhibited both phases of the oxidative burst and suppressed wound-regulated changes in 9/28 extracellular proteins. DPI infiltrated 15 min after wounding only partially inhibited early O-2(-) production and was ineffective in suppressing wound-related changes in these proteins. This strongly suggests that in wounded Medicago, rapid O-2(-) is required for mobilizing the downstream (3-6 h), differential expression of several extracellular proteins. Further studies with DPI and exogenous sources of ROS supported the regulation of these proteins within early, wound-related ROS-signaling events. The study forms the basis for associating wound-related changes in the apoplast proteome with ROS-dependent and ROS-independent pathways. Proteins mobilized within the ROS-dependent pathway were largely ionically bound to cell walls and included SODs, peroxidases and germin-like proteins, suggesting their involvement within wound-activated, ROS regulatory loops.
Original languageUnknown
Pages (from-to)2298-2309
JournalJournal Of Proteome Research
Issue number5
Publication statusPublished - 1 Jan 2009

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