TY - JOUR
T1 - Assessment of the BD MGIT TBc identification test for the detection of mycobacterium tuberculosis complex in a network of mycobacteriology laboratories
AU - Machado, Diana
AU - Ramos, Jorge
AU - Couto, Isabel
AU - Cadir, Nureisha
AU - Narciso, Inácio
AU - Coelho, Elizabeth
AU - Viegas, Sofia
AU - Viveiros, Miguel
N1 - WOS:000330695600001
PY - 2014
Y1 - 2014
N2 - We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations in mpb64 gene are present or some M. bovis BCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.
AB - We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations in mpb64 gene are present or some M. bovis BCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.
UR - http://www.scopus.com/inward/record.url?scp=84893728808&partnerID=8YFLogxK
UR - https://www.hindawi.com/journals/bmri/2014/398108/
U2 - 10.1155/2014/398108
DO - 10.1155/2014/398108
M3 - Article
C2 - 24587985
AN - SCOPUS:84893728808
VL - 2014
SP - 398108
EP - 398115
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
M1 - 398108
ER -