Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments

Nora Maruniakova, Attila Kadasi, Alexander V. Sirotkin, Agnieszka Leśniak, Ana M M Ferreira, Jozef Bulla, Adriana Kolesarova

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Assessment of A-trichothecene mycotoxins (T-2 and HT-2 toxins) effect combined with growth factor IGF-I, and the metabolic hormones leptin and ghrelin on progesterone secretion by rabbit ovarian fragments was studied. Rabbit ovarian fragments were incubated without (control group) or with T-2/HT-2 toxin, or their combinations with insulin-like growth factor I (IGF-I), leptin or ghrelin at various concentartions for 24 h. Secretion of progesterone was determined by ELISA. First, T-2 toxin and HT-2 toxins at all doses used (0.01, 0.1, 1, 10, and 100 ng mL–1) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Second, T-2 toxin but not HT-2 toxin combined with IGF-I was shown to be potential regulator of progesterone secretion in rabbit ovarian fragments. T-2 toxin at all doses used (0.01; 0.1; 1; 10; and 100 ng mL–1) combined with IGF-I (at dose 100 ng mL–1) significantly (P < 0.05) decreased progesterone secretion by rabbit ovarian fragments. Third, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL–1) combined with leptin (at dose 1000 ng mL–1) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Furthermore, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL–1) combined with ghrelin (500 ng mL–1) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Results in this study showed that trichothecene as T-2 toxin combined with IGF-I but not HT-2 toxin was able to decrease progesterone secretion in rabbit ovarian fragments in vitro. Experimental results of T-2 and HT-2 toxins combined with leptin and ghrelin did not confirm ability to modulate progesterone secretion by ovarian fragments in rabbits.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalJournal of Environmental Science and Health, Part B
Volume50
Issue number2
DOIs
Publication statusPublished - 1 Feb 2015

Keywords

  • ghrelin
  • HT-2 toxin
  • IGF-I
  • leptin
  • ovary
  • Progesterone
  • rabbit
  • T-2 toxin

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