@inbook{00519f52e4424382aec29552ba5740e9,
title = "Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway",
abstract = "Protein glutathionylation is a posttranslational process that regulates protein function in response to redox cellular changes. Furthermore, carbon monoxide-induced cellular pathways involve reactive oxygen species (ROS) signaling and mitochondrial protein glutathionylation. Herein, it is described as a technique to assess mitochondrial glutathionylation due to low concentrations of CO exposure. Mitochondria are isolated from cell culture or tissue, followed by an immunoprecipitation assay, which allows the capture of any glutathionylated mitochondrial protein using a specific antibody coupled to a solid matrix that binds to glutathione antigen. The precipitated protein is further identified and quantified by immunoblotting analysis.",
keywords = "Carbon monoxide, Glutathione, Glutathionylation, Immunoprecipitation, Mitochondria",
author = "Almeida, {Ana S.} and Cl{\'a}udia Figueiredo-Pereira and Vieira, {Helena L.A.}",
note = "Funding Information: This work was supported by the Portuguese Funda??o para a Ci?ncia e Tecnologia (FCT) for the grants FCT-ANR/ NEUNMC/0022/2012 and UID/Multi/04462/2013, I&D 2015-2020 iNOVA4Health ? Programme in Translacional Medicine; and for ASA?s SFRH/BD/78440/2011 and CFP?s SFRH/ BD/106057/2015 fellowships. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
doi = "10.1007/978-1-0716-1266-8_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "249--257",
booktitle = "Methods in Molecular Biology",
address = "United States",
}