Assessment of mitochondrial protein glutathionylation as signaling for CO pathway

Ana S. Almeida; Helena L A Vieira

doi:10.1007/978-1-4939-2257-4_30

Assessment of mitochondrial protein glutathionylation as signaling for CO pathway

Ana S. Almeida, Helena L A Vieira

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Protein glutathionylation is a posttranslational process that regulates protein function in response to redox cellular changes. Furthermore, carbon monoxide-induced cellular pathways involve reactive oxygen species (ROS) signaling and mitochondrial protein glutathionylation. Herein, it is described a technique to assess mitochondrial glutathionylation due to low concentrations of CO exposure. Mitochondria are isolated from cell culture or tissue, followed by an immunoprecipitation assay, which allows the capture of any glutathionylated mitochondrial protein using a specific antibody coupled to a solid matrix that binds to glutathione antigen. The precipitated protein is further identified and quantified by immunoblotting analysis.

Original language	English
Title of host publication	Probing Mitochondrial Function
Publisher	Springer New York
Pages	343-350
Number of pages	8
Volume	1
ISBN (Electronic)	9781493922574
ISBN (Print)	9781493922567
DOIs	https://doi.org/10.1007/978-1-4939-2257-4_30
Publication status	Published - 28 Jan 2015

Keywords

Carbon monoxide
Glutathione
Glutathionylation
Immunoprecipitation
Mitochondria

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10.1007/978-1-4939-2257-4_30

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Almeida, A. S., & Vieira, H. L. A. (2015). Assessment of mitochondrial protein glutathionylation as signaling for CO pathway. In Probing Mitochondrial Function (Vol. 1, pp. 343-350). Springer New York. https://doi.org/10.1007/978-1-4939-2257-4_30

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