Assessment of gamma oryzanol variability, an attractive rice bran bioactive compound

M. Manuela Lageiro, Ana Castanho, Cristiana Pereira, Ricardo C. Calhelha, Isabel C.F.R. Ferreira, Carla Brites

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Gamma-oryzanol is a compound present on rice bran, which has been studied for its bioactive properties, including its cytotoxic activity. The gamma-oryzanol biosynthesis occurs on the lipidic membrane of the plant and can be influenced by edaphoclimatic grown conditions. Two Portuguese rice varieties (Ceres and Macarico) from 6 growing environments were analysed in order to explore the influence of the growing conditions on the gamma-oryzanol content, its constituents, and cytotoxic effect against 4 human tumour cell lines (NCI-H460, HeLa, HepG2, and MCF-7). The overall variability of gamma-oryzanol concentration was from 1.56 g/Kg to 3.19 g/Kg, Ceres reveals higher values than Macarico; those concentrations also varied with the growing environment. The environment was also determinant for the individual concentration of the 3 gamma-oryzanol compounds (cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate). A cytotoxic effect was observed for all the tested tumour cell lines and also varied with the growing environment.

Original languageEnglish
Article number2056
Pages (from-to)38-46
Number of pages9
JournalEmirates Journal of Food and Agriculture
Issue number1
Publication statusPublished - 1 Jan 2020


  • Cytotoxicity
  • Gamma-oryzanol
  • HPLC
  • Rice bran


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