@article{c89448b033cc427c82ef3ffaf82191e4,
title = "Assessing diffusion in enzyme loaded sol-gel matrices",
abstract = "Pulsed field gradient spin echo high resolution magic angle spinning nuclear magnetic resonance spectroscopy is a powerful technique to characterize confined biosystems. We used this approach to assess the diffusion of solvent and reaction species within sol-gel matrices differing in enzyme loading.",
keywords = "UNUSUAL FLUORESCENCE BEHAVIOR , NMR SELF-DIFFUSION, ANGLE-SPINNING NMR , SINGLE TRYPTOPHAN , CUTINASE , IMMOBILIZATION , SPECTROSCOPY , LIQUIDS, DEPOLARIZATION , BIOSENSORS",
author = "Gustavo Barreira and Ferreira, {Ana S. D.} and Pedro Vidinha and Cabral, {Joaquim M. S.} and Martinho, {Jos{\'e} M. G.} and Lima, {Jo{\~a}o Carlos} and Cabrita, {Eurico J.} and Susana Barreiros",
note = "This work has been supported by Fundacao para a Ciencia e a Tecnologia (FCT/MEC) through projects/grants PEst-C/EQB/LA0006/2013, PTDC/QUI/64744/2006, SFRH/BD/34800/2007 (GB), SFRH/BPD/41546/2007 (PV) and SFRH/BPD/88144/2012 (ASDF), and by FEDER. The NMR spectrometers are part of the National NMR Network (RNRMN) and are funded by FCT/MEC (project RECI/BBB-BQB/0230/2012). We thank Prof. Luis Fonseca and Mario Fonseca for the production of cutinase, Marta Corvo for the acquisition of HR-MAS spectra, Dr Alan Phillips for performing enzyme particle size measurements, and Macherey-Nagel for the gift of Polygoprep silica particles.",
year = "2014",
doi = "10.1039/c4ra01620c",
language = "English",
volume = "4",
pages = "25099--25105",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "RSC - Royal Society of Chemistry",
number = "48",
}