Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Pedro Bule, Victor D Alves, Vered Israeli-Ruimy, Ana L Carvalho, Luís M A Ferreira, Steven P Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M G A Fontes

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.

Original languageEnglish
Article number759
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 7 Apr 2017

Keywords

  • SINGLE BINDING MODE
  • CRYSTAL-STRUCTURE
  • DEGRADING ENZYMES
  • CELL-SURFACE
  • PROTEIN
  • QUALITY
  • SPECIFICITY

Fingerprint Dive into the research topics of 'Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes'. Together they form a unique fingerprint.

  • Cite this

    Bule, P., Alves, V. D., Israeli-Ruimy, V., Carvalho, A. L., Ferreira, L. M. A., Smith, S. P., ... Fontes, C. M. G. A. (2017). Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes. Scientific Reports, 7(1), [759]. https://doi.org/10.1038/s41598-017-00919-w