Assembling a Low-volume Biofuel Cell on a Screen-printed Electrode for Glucose Sensing

Jana M. Becker, Anna Lielpetere, Julian Szczesny, Adrian Ruff, Felipe Conzuelo, Wolfgang Schuhmann

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


An enzymatic biofuel cell is integrated on a screen-printed electrode as a basis for a self-powered biosensor. A glucose/O2 biofuel cell consisting of a pyrroloquinoline quinone-dependent glucose dehydrogenase embedded within an Os-complex modified redox polymer bioanode to oxidize glucose and a non-limiting bilirubin oxidase-based gas diffusion biocathode in the direct-electron transfer regime for the reduction of O2 showed a glucose-dependent current and power output. For full integration on a single screen-printed electrode, a miniaturized agar salt bridge was introduced between the two bioelectrodes to ensure operation of the assembly in a two-compartment configuration with each electrode operating at optimal conditions.

Original languageEnglish
Pages (from-to)1629-1637
Number of pages9
Issue number10
Publication statusPublished - Oct 2022


  • biofuel cell
  • enzyme electrodes
  • glucose
  • screen-printed electrode
  • self-powered sensor


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