Aspergillus prevalence in air conditioning filters from vehicles

Taxis for patient transportation, forklifts, and personal vehicles

Carla Viegas, Ricardo Moreira, Tiago Faria, Liliana Aranha Caetano, Elisabete Carolino, Anita Quintal Gomes, Susana Viegas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The frequency and importance of Aspergillus infections is increasing worldwide. This study aimed to assess the occupational exposure of forklifts and taxi drivers to Aspergillus spp. Nineteen filters from air conditioning system of taxis, 17 from forklifts and 37 from personal vehicles were assessed. Filters extract were streaked onto MEA, DG18 and in azole-supplemented media. Real-time quantitative PCR amplification of selected Aspergillus species-complex was also performed. Forklifts filter samples presented higher median values. Aspergillus section Nigri was the most observed in forklifts filters in MEA (28.2%) and in azole-supplemented media. DNA from Aspergillus sections Fumigati and Versicolores was successfully amplified by qPCR. This study enlightens the added value of using filters from the air conditioning system to assess Aspergillus spp. occupational exposure. Aspergillus azole resistance screening should be included in future occupational exposure assessments.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalArchives of Environmental and Occupational Health
Volume74
Issue number6
DOIs
Publication statusAccepted/In press - 5 Jul 2018

Fingerprint

Transportation of Patients
Air Filters
Air Conditioning
taxis
air conditioning
Aspergillus
Air conditioning
filter
occupational exposure
Azoles
Occupational Exposure
species complex
amplification
vehicle
DNA
Amplification
Real-Time Polymerase Chain Reaction
Screening

Keywords

  • air conditioning filter
  • Aspergillus azole resistance screening
  • Aspergillus spp
  • forklifts
  • occupational exposure assessment
  • taxis

Cite this

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abstract = "The frequency and importance of Aspergillus infections is increasing worldwide. This study aimed to assess the occupational exposure of forklifts and taxi drivers to Aspergillus spp. Nineteen filters from air conditioning system of taxis, 17 from forklifts and 37 from personal vehicles were assessed. Filters extract were streaked onto MEA, DG18 and in azole-supplemented media. Real-time quantitative PCR amplification of selected Aspergillus species-complex was also performed. Forklifts filter samples presented higher median values. Aspergillus section Nigri was the most observed in forklifts filters in MEA (28.2{\%}) and in azole-supplemented media. DNA from Aspergillus sections Fumigati and Versicolores was successfully amplified by qPCR. This study enlightens the added value of using filters from the air conditioning system to assess Aspergillus spp. occupational exposure. Aspergillus azole resistance screening should be included in future occupational exposure assessments.",
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Aspergillus prevalence in air conditioning filters from vehicles : Taxis for patient transportation, forklifts, and personal vehicles. / Viegas, Carla; Moreira, Ricardo; Faria, Tiago; Caetano, Liliana Aranha; Carolino, Elisabete; Gomes, Anita Quintal; Viegas, Susana.

In: Archives of Environmental and Occupational Health, Vol. 74, No. 6, 05.07.2018, p. 341-349.

Research output: Contribution to journalArticle

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